27 



germinating spores were transferred directly from the agar of these 

 cultures to the usual media. In every case the typical gray sterile 

 mycelium, similar in every way to that used in the inoculations of 

 the preceding year, resulted. This when inoculated into the berries 

 produced the usual spots from which it was reisolated, and there can 

 thus be no doubt as to the identit}^ of the organism producing this 

 spot. An attempt was made to show that the spot on the berry could 

 be produced by spores from the leaf spots. When transferred di- 

 rectly from the leaves to the berries no infection resulted. Later 

 pure cultures from the spores of the Cercospora of the leaf were 

 obtained, as in the case of that of the berry. The resulting growth, 

 which was similar in every way to that in cultures of the berry 

 fungus, when inoculated into the berries, gave positive results, typi- 

 cal Cercospora spores later developing. 



In conclusion it may be said that the spot of the berries produced 

 by Cercospora coffeicola^ which besides interfering with the prepa- 

 ration by causing the flesh to dry and adhere to the berry also injures 

 the grain to some extent, may be largely prevented by the use of suffi- 

 cient shade, as, for example, that of sufficient density to prevent the 

 growth of grasses other than palmilla. Such shade is distinctly 

 favorable to the production of coffee free from inferior shrunken 

 grains. 



MISCELLANEOUS DISEASES. 



Of less importance but perhaps worth mentioning in any general 

 discussion of coffee diseases are the " zoned " leaf spot and a root 

 and trunk disease. The leaf spot is characterized by its tendency 

 to develop concentric rings, such rings being sometimes incomplete 

 at the outside of the spot, and often beginning as entirely separate 

 spots which increase until united with the central mass (PI. IV). 

 On the underside of the older spots a thin white mold appears after 

 a while, which is condensed or massed in places. This bears the 

 numerous spores of the fungus {C&phalosporium sp.) (PI. VIII, C.) 

 which has been shown to be the cause of the spot by isolation from 

 the beginning spots and by inoculations from pure culture. The 

 disease is common among the best coffee, seeming to prefer the young 

 and well-shaded leaves, and all the various species and varieties 

 cultivated at this station are subject to attack, though it is most 

 common on the "Ceylon Hybrid." It has been noticed on other 

 plantations but is nowhere abundant. 



Another disease is easily recognized by the smaller diameter of 

 the affected part of the trunk owing to the bark drying up and 

 shrinking (PL V). After being diseased for a long time the bark 

 falls away leaving the wood exposed and the calloused outgrowths 

 at the edge of the healthy bark. If the diseased bark is cut away 



