Notes on certain Blood- Inhabiting Protozoa. 235 



karyosomes and through the strand wliich at this stn^e 

 joins them. Some specimens suggest that the red strand 

 between the two daughter karyosomes has disappeared, and 

 left the blue staining central line. The remaining chromatin 

 at this stage may be arranged in various ways (Figs. 5, 6, 7, 8). 

 It may either be scattered over an irregular fine reticulum 

 {i.e., the chromatic part of the nucleus is still in the resting 

 condition), or may form separate, more or less rod-shaped 

 chromosomes. It may form cross bars running from one 

 side of the nucleus to the other, or bars attached to the 

 central line, and passing from it to the periphery, or the 

 chromatin may already be arranged in the irregularly shaped 

 caps at either end round the karyosomes. 



A later stage of this process shows the two karyosomes 

 completely separated : the line is now interrupted in the 

 middle, and passes from the blepharoplast to one karyosome, 

 and from the other, i.e., the anterior karyosome, to the tip of 

 the body (Figs. 1, 2). At division of the plasma the line 

 apparently divides, but the process is obscure. This much, 

 however, is certain, that specimens are found in a late stage 

 of division when blepharoplast, nucleus, and membrane are 

 already divided (or sometimes a little earlier), which show two 

 central lines passing forwards, one in each daughter individual. 



In many specimens that part of the line which lies 

 anterior to the nucleus bears a number of thickenings, stain- 

 ing from violet to reddish- violet or even red ; sometimes 

 bars can be seen to pass out from these either on one side or 

 on both (Figs. 9, 7, 8, 5). 



These appearances were for a time incomprehensible, until 

 a number of forms were met with which offered an explana- 

 tion. Trypanosomes were found (Figs. 11, 6, 3) which showed 

 a spiral band of chromatin-staining substance (giving a clear 

 red reaction with the modified Eomanowski) in the pre- 

 nuclear part of the animal. When this band is present the 

 central line is often only to be seen in the post-nuclear part, 

 but specimens are found which show both the spiral band 

 and the line. The line and the band are really two separate 

 structures, although they seem to have a certain amount of 

 connection with one another. The spiral is continued some- 



