Notes on certain Blood- Inhabiting Protozoa. 247 



Explanation of Figures. 



All the figures are from preparations stained with modifications of the 

 Romanowski method, with the exception of Fig. 14, which is stained with 

 polychrome methylene blue. The figures are all Camera lucida drawings, 

 made under the two millimetre apochromatic oil immersion lens, by Zeiss, 

 and the eighteen compensating eye-piece. The drawing was made at the 

 level of the table. 



Figs. 1 to 12 represent Try2janosoma brucei. 

 Fig. 1. The karyosome is divided into two parts: the chromatin is repre- 

 sented by rods arranged in an oval. 

 Division stage showing central line. 



Spiral in pre-nuclear part, and central line in post-nuclear part. 

 Autosynthesis (?) of the nucleus. 

 Central line passing through the nucleus ; it shows thickening in 



pre-nuclear part. The karyosome has divided. 

 Central line and spiral in pre-nuclear part. 

 Chromatin arranged in cross bars ; central line present. 

 Chromatin of the nucleus arranged in cross bars. This shows the 

 vacuole and granule at the anterior extremity of the central 

 line. 

 Fig. 9. The central line shows thickenings and cross bars in pre-nuclear 



part. 

 Fig. 10. Nucleus, with chromatin in bars. 



Fig. 11. The pre-nuclear spiral band is continued into the nucleus. 

 Fig. 12. Spiral arrangement of the chromatin in the nucleus. 



Figs. 13 to 23 represent the endocorpuscular parasite found in 

 Pleuronectes platessa and Pleuronectes flesus. 



Fig. 13. This shows the eosinophile body and the centrosome: the arrange- 

 ment of the chromatin in this specimen is obscure. 



Fig. 14. Specimen stained with polychrome methylene blue, eosinophile 

 body unstained, centrosome present: nucleus shows the karyo- 

 some and eight somewhat irregularly -shaped chromosomes. 



Fig. 15. Karyosome elongated : the chromosomes appear to be dividing. 



Fig. 16. Eight chromosomes, karyosome : the nuclear membrane is absent. 



Fig. 17. This shows the centrosome with radiations passing from it; it is 

 lying in the protoplasm, and not adjacent to the eosinophile body. 



Fig. 18. Large granular form, with compact nucleus; the separate chromo- 

 somes are not clearly defined. 



Fig. 19. Smaller form, with large nucleus ; probably intermediate stage. 



Fig. 20. Division of nucleus : nucleus nearest eosinophile body shows eight 

 chromosomes. 



Fig. 21. Much the same stage as Fig. 18, but here centrosome apparently 

 inside the eosinophile body. 



Fig. 22. Form with a large and less compact nucleus; the protoplasm 

 shows the eosinophile body and a vacuole. 



Fig. 23. Division of nucleus suggesting reduction of chromatin : there are 

 apparently only four chromosomes in each nucleus. 

 VOL. XVI. Z 



