Ficrther Notes on the Chemical Com2')osition of Ensilage. 281 



Tliese results calculated free from the moisture gave : — 

 Analysis of Ensilage. 

 {Moist sample ccdculatcd free from moisture.) 



Ether extract, 7775 



Albuminous compounds, etc., ..... 14 "402 



Curboliydrates, etc., 45 949 



Woody fibre, 19-478 



Ash 12-394 



Total nitrogen, 2-304% 



Phosphates in ash, . . . . . . . l'4997o 



After drying a portion, the total nitrogen then found to 

 be present was equal to 1*893 %, showing a loss of nitrogen 

 during the drying process equal to 0*411 ^ of the dried 

 sample. At this stage of the inquiry, the nitrogen deter- 

 minations yielded the following results : — 



684% 

 304% 

 893% 

 831% 

 411% 



Nitrogen in moist sample, ..... 

 Which calculated to water-free sample is equal to . 2 

 Nitrogen in dried sample, ..... 1 



= Albumenoids, . . . . . .11 



Nitrogen lost during drying process, ... 



= Ammonia, ....... 



On distilling a portion of the fresh sample with water, a 

 distillate was obtained which was acid in character. This 

 was considered as proof that no free ammonia was contained 

 in the sample, a fact rendered evident by the acid character 

 of the ensilage. The distillate was afterwards treated with 

 sodic carbonate and re-distilled, when a quantity of ammonia 

 was found in the distillate equal to 0"481 ^ of the original 

 sample. The results for the volatile ammonia then stood 

 thus : — 



Ammonia = Nitrogen lost on drying original sample, -49970 

 Ammonia = Nitrogen obtained by distillation = vola- 

 tile ammonia, -48170 



Ammonia = Volatile nitrogen not accounted for, . 0-0187o 



Containing nitrogen, -01570 



The residual material left in the retort after the first dis- 

 tillation was found to be still acid, but on treatment with 

 sodic carbonate yielded a distillate containing nitrogen equal 



