﻿GO 
  

  

  up 
  the 
  interstices 
  and 
  forming 
  a 
  layer 
  around 
  the 
  outside 
  or 
  

   periphery. 
  This 
  change 
  continues 
  until 
  the 
  outer 
  portion 
  

   assumes 
  a 
  mottled 
  granular 
  appearance. 
  One 
  side 
  — 
  and 
  it 
  

   is 
  always 
  on 
  the 
  side 
  — 
  then 
  assumes 
  a 
  lighter 
  shade, 
  becomes 
  

   very 
  evenly, 
  fmel}' 
  granular, 
  and 
  forms 
  a 
  less 
  or 
  greater 
  

   protuberance 
  from 
  the 
  vitellus. 
  Fig. 
  3, 
  PL 
  I. 
  This 
  form 
  

   continues 
  with 
  more 
  or 
  less 
  distinctness, 
  sometimes 
  assu- 
  

   ming 
  the 
  fissural 
  condition 
  of 
  segmenting 
  or 
  impregnated 
  

   eggs, 
  until 
  the 
  yolk 
  gradually 
  turns 
  white, 
  disintegrated, 
  

   and 
  (lies. 
  

  

  I 
  was 
  unable 
  to 
  find 
  among 
  the 
  oil-globules 
  of 
  the 
  virellus, 
  

   any 
  germinal 
  vesicle 
  with 
  its 
  germinal 
  dot, 
  although 
  1 
  

   opened 
  a 
  great 
  number 
  of 
  ova, 
  and 
  examined 
  the 
  contents 
  

   with 
  Zeiss 
  ocular 
  4 
  and 
  objective 
  D, 
  giving 
  400 
  diameters 
  

   enlargement. 
  I 
  did 
  find, 
  however, 
  in 
  most 
  of 
  (he 
  ova, 
  a 
  

   small 
  bit 
  of 
  granular 
  tissue, 
  which 
  may 
  represent 
  the 
  germi- 
  

   nal 
  vesicle, 
  or 
  more 
  properly, 
  perhaps, 
  the 
  blastodermic 
  titi- 
  

   sue. 
  I 
  am 
  inclined 
  to 
  flunk 
  that 
  the 
  germinal 
  vesicle 
  

   undergoes 
  a 
  differentiation 
  during 
  the 
  period 
  of 
  ripening 
  of 
  

   the 
  egg 
  ; 
  and 
  although 
  no 
  true 
  segmentation 
  takes, 
  place 
  

   until 
  after 
  contact 
  with 
  the 
  male 
  element, 
  yet 
  a 
  granulation 
  

   takes 
  place 
  whicli 
  changes 
  the 
  vesicle 
  with 
  its 
  contents, 
  into 
  

   blastodermic 
  tissue, 
  ready 
  to 
  be 
  segmented 
  and 
  still 
  furthei- 
  

   differentiated 
  upon 
  contact 
  with 
  the 
  male 
  element, 
  or 
  without 
  

   contact, 
  to 
  disintegrate. 
  It 
  is 
  highly 
  probable 
  that 
  in 
  an 
  

   earlier 
  stage 
  than 
  I 
  have 
  examined, 
  the 
  vesicle, 
  with 
  its 
  

   contained 
  germinal 
  dot, 
  can 
  be 
  found. 
  

  

  Spermatozoa 
  . 
  

  

  The 
  milt 
  in 
  the 
  male 
  occupies 
  relatively 
  less 
  bulk 
  than 
  

   the 
  spawn 
  in 
  the 
  female 
  ; 
  and 
  the 
  males 
  can 
  thus 
  be 
  desig- 
  

   nated 
  from 
  the 
  females, 
  during 
  the 
  spawning 
  season, 
  by 
  

   their 
  more 
  slender 
  form, 
  there 
  being 
  very 
  little 
  of 
  what 
  is 
  

   vulgarly 
  known 
  as 
  pot-bell 
  led 
  ness 
  about 
  them. 
  

  

  