﻿It) 
  

  

  water 
  must 
  be 
  kept 
  free 
  from 
  fragments 
  of 
  the 
  various 
  organs 
  

   of 
  the 
  adult, 
  as 
  these 
  would 
  soon 
  decay 
  and 
  destroy 
  the 
  em- 
  

   bryos, 
  and 
  it 
  is 
  therefore 
  important 
  to 
  remove 
  them 
  as 
  com- 
  

   pletely 
  as 
  possible. 
  After 
  the 
  mass 
  has 
  been 
  chopped 
  up 
  a& 
  

   fine 
  as 
  possible, 
  fill 
  up 
  the 
  watch 
  crj^stal 
  with 
  fresh 
  sea 
  water, 
  

   stir 
  it 
  up, 
  and 
  then 
  allow 
  it 
  to 
  run 
  into 
  one 
  of 
  the 
  smallest 
  

   beakers, 
  which 
  has 
  been 
  nearly 
  filled 
  with 
  sea 
  water. 
  As 
  the 
  

   water 
  runs 
  out 
  of 
  the 
  watch 
  crystal, 
  be 
  careful 
  to 
  allow 
  as 
  

   few 
  of 
  the 
  fragments 
  as 
  possible 
  to 
  run 
  with 
  it. 
  

  

  Now 
  fill 
  up 
  the 
  watch 
  crystal 
  with 
  water 
  again, 
  and 
  stir 
  

   and 
  pour 
  oft" 
  as 
  before, 
  and 
  repeat 
  the 
  process 
  until 
  nearly 
  all 
  

   of 
  the 
  male 
  fluid 
  has 
  been 
  w^ashed 
  out 
  of 
  the 
  fragments 
  and 
  

   poured 
  into 
  the 
  beaker. 
  Stir 
  the 
  contents 
  of 
  the 
  beaker 
  for 
  a 
  

   short 
  time, 
  and 
  then 
  allow 
  it 
  to 
  stand 
  about 
  five 
  minutes, 
  to 
  

   allow 
  any 
  fragments 
  to 
  settle 
  to 
  the 
  bottom, 
  then 
  pour 
  the 
  

   fluid, 
  which 
  should 
  be 
  quite 
  milky, 
  into 
  another 
  small 
  beaker, 
  

   leaving 
  behind, 
  to 
  be 
  thrown 
  away, 
  any 
  particles 
  which 
  may 
  

   have 
  settled 
  to 
  the 
  bottom. 
  The 
  male 
  cells 
  retain 
  their 
  full 
  

   vitality 
  for 
  several 
  hours 
  after 
  they 
  have 
  been 
  mixed 
  with 
  sea 
  

   water, 
  so 
  the 
  beaker 
  may 
  be 
  set 
  aside 
  to 
  wait 
  until 
  the 
  eggs 
  

   are 
  ready. 
  The 
  eggs 
  swell 
  up 
  and 
  break 
  to 
  pieces 
  within 
  a 
  

   very 
  few 
  minutes 
  after 
  they 
  are 
  mixed 
  with 
  water, 
  unless 
  they 
  

   are 
  fertilized 
  at 
  once, 
  so 
  it 
  is 
  much 
  better 
  to 
  add 
  the 
  eggs 
  to 
  

   a 
  previously 
  prepared 
  mixture 
  of 
  male 
  cells 
  and 
  water 
  than 
  it 
  

   is 
  to 
  put 
  the 
  eggs 
  into 
  the 
  water 
  to 
  wait 
  until 
  the 
  male 
  fluid 
  

   is 
  got 
  ready. 
  

  

  Taking 
  now 
  one 
  of 
  the 
  females, 
  remove 
  and 
  chop 
  up 
  the 
  

   ovary 
  in 
  the 
  same 
  way 
  in 
  another 
  watch 
  crystal, 
  observing 
  

   the 
  same 
  precautions 
  in 
  removing 
  all 
  portions 
  of 
  the 
  body. 
  

   Fill 
  the 
  watch 
  glass 
  with 
  water, 
  and 
  stir 
  and 
  pour 
  oflF 
  into 
  the 
  

   beaker 
  as 
  before, 
  giving 
  the 
  contents 
  of 
  the 
  beaker 
  a 
  good 
  

   stirring 
  after 
  each 
  lot 
  of 
  eggs 
  is 
  added, 
  in 
  order 
  to 
  diffuse 
  

   them 
  through 
  the 
  water 
  at 
  once, 
  and 
  thus 
  insure 
  the 
  speedy 
  

   contact 
  of 
  each 
  of 
  them 
  with 
  some 
  of 
  the 
  male 
  cells. 
  

  

  Fill 
  the 
  crystal 
  with 
  water 
  again, 
  and 
  stir 
  and 
  pour 
  off, 
  

   and 
  repeat 
  until 
  all 
  the 
  eggs 
  have 
  been 
  washed 
  out 
  of 
  the 
  

   fragments 
  of 
  the 
  ovary. 
  

  

  