Pammel — Anatomical Characters of Seeds of Leguminosae. 103 



under the exterior wall. This is followed by a wide clear 

 band which extends above the enlarged cell-cavity in the 

 upper third of the cell. On the addition of chlor-iodide 

 of zinc the whole cell-wall colors yellowish-brown, the lower 

 part nauch darker, the light line taking on a very pale color. 

 The wide band turns blue except the narrow light line, which 

 remains nearly colorless. Phloroglucin causes the narrowlight 

 line to color immediately. It also colors the cell-walls below 

 the cell-cavity, the wide clear band remaining nearly color- 

 less. The narrow light line and the lower part of the Mal- 

 pighian cell are lignified, while the broad band is made up 

 mostly of cellulose. 



Cucurbitiiceae, The following writers have studied the testa 

 of this order. Targioni-Tozzetti (257) ; Straudmark (254); 

 V. Hoehnel (379); Fickel (332); Godfrin (71); Hartwich 

 (368); Harz (99, 2 : 767-824. /. 39-45); Mattirolo (401, 

 20); Michelis (410); Junowicz (128) ; and Holfert (116). 



In jSicyos angulaius, the whole upper part above the en- 

 larged cell-cavity is lighter in color than the remainder of the 

 Malpighian cell, except a narrow zone in the lower part of the 

 cell. It is easy to distinguish three light lines. Junowicz 

 found two light lines in Luffa acutangula. I have verified this 

 for the species. Mattirolo, who studied the genus carefully, 

 finds a very distinct reaction for lignin with the phloroglucin 

 test. In Luffa acutangula the Malpighian cells color very 

 rapidly with phloroglucin and hydrochloric acid, the light 

 line less rapidly. In sections where the reaction has taken 

 place with this reagent, the light line can be made out as a 

 somewhat brighter band. In Luffa the cells above the Mal- 

 pighian layer are also lignified, as well as the cell- walls of the 

 nutrient layer. 



In jSicyos angulaius the phloroglucin test acts rapidly on 

 the Malpighian cells, except the two upper light lines. These 

 resist its action except for a very slight coloration, so that a 

 nearly hyaline band may be seen stretching across the section, 

 and this after the section has been subjected to this treatment 

 for half an hour. Chlor-iodide of zinc colors the Malpighian 

 cell a yellowish-brown except tl^e narrow light line in the 

 upper part of the cell. This colors a pale yellow and ulti- 



