Pammel — Anatomical Characters of Seeds of Leguminosae. 159 



make up nearly half of the thickness of the testa and 

 endosperm. 



Malpighian, Cells 56 /u, in length. Cuticle an even con- 

 tinuous layer, followed by the cuticularized zone ; the narrow 

 light line lies below the latter. Several pore-canals extend 

 into the walls for nearly one-third of the length of the cell. 

 Cell-cavity large at the base, containing protein grains and a 

 chromatophore, the latter variable as to position. 



Osteosclerid, Cells are thick-walled ; upper and lower bars 

 nearly equal. Intercellular spaces nearly square. Walls color 

 blue with chlor-iodide of zinc. 



Nutrient. Layer much compressed, variable as to thick- 

 ness. The cells are rather thick-walled; only a small amount 

 of pigment, tannin and some protein grains present; vascu- 

 lar elements occur in the lower part. Cell- walls color blue 

 with chlor-iodide of zinc. 



Endosperm, Aleurone layer nearly isodiametric, thick- 

 walled, containing protein. This layer is followed by two or 

 three rows of thick-walled cells with pore-canals. Walls 

 color blue with chlor-iodide of zinc. The internal layer of 

 the reserve cellulose consists of thick-walled, elongated cells 

 with narrow lumen. Cells contain fat and protein. 



Embryo. Cells of the first row with exterior walls thick- 

 ened, smaller than those below; the interior cells are irregular 

 with intercellular spaces ; walls color blue with chlor-iodide of 

 zinc. All of the cells of the embryo contain fat and protein, 

 but little or no starch, and an abundance of compound crys- 

 tals of oxalate of lime. Two rows of well-developed palisade 

 parenchyma cells occur on the upper surface of seed during 

 germination, or on the side next to the endosperm ; the first row 

 more compactly arranged than the rows below ; the third is 

 much interrupted because of the lysigenetic reservoirs. With a 

 hand-lens one is able to readily distinguish spherical glistening 

 bodies, somewhat irregularly distributed; higher magnifica- 

 tion shows that these glistening bodies are surrounded by the 

 parenchyma of the cotyledons. In some places these cells are 

 shorter than the remaining parenchyma. In a longitudinal 

 section these bodies are about of the same diameter. The 

 development has not been studied, but in some sections it 



