Pammel — Anatomical Characters of Seeds of Leguminosae. 193 



lowed by ordinary parenchyma. Procambial vessels not lig- 

 nified. Cells contain an abundance of fat, protein, and large, 

 stratified starch grains. 



Phaseolus multiflorus, Willd. 



PI. XXL f. 2-2g. 



A full account of this seed is given by Mattirolo and Bus- 

 calioni. Tschirch and Oesterle, Harz, Holfert, and Haber- 

 landt also treat the anatomy of the seed briefly. A general 

 account is also given by Lubbock, and by Sachs, who studied 

 its germination. 



Holfert distinguishes five layers of the testa : ( 1 ) Mal- 

 pighian, (2) osteosclerid, (3) obliterated nutrient layer, (4) 

 star-shaped parenchyma, (5) obliterated star-shaped paren- 

 chyma (verfilztj. The testa is spotted, much larger than 

 in P. perennis, laterally 580 fi thick, towards the edges 770 fi 

 thick. 



Malpighian. The cells are 84 fi long. Cuticle smooth; 

 cuticularized layer not strongly developed ; light line runs 

 close under the latter ; pores project into the wall below the 

 light line. Cell-cavity large at the base, narrowed rather 

 abruptly above. Walls of some of the cells as well as the 

 cavity contain a bluish pigment, especially in the lower por- 

 tion. The walls, cuticularized layer, and light line color 

 blue with chlor-iodide of zinc. 



Osteosclerid. Cell-walls thickened, longitudinally striated. 

 Cells I-shaped, with elongated intercellular spaces. Cells 

 contain some pigment. 



N'lUrient. Layer differentiated into two parts ; cells of the 

 upper portion thin-walled, loosely arranged, with numerous 

 intercellular spaces ; but little pigment present. Cells of the 

 lower part narrower, thicker-walled, with more pigment, and 

 tannin. Vascular elements occur in this part of the nutrient 

 layer ; these are also pigmented. The walls of the vessels 

 give the reaction for lignin. The walls of the other cells 

 of the nutrient layer color blue with chlor-iodide of zinc. 



Mycotic. In cross-section the cells are closely packed 

 and would pass for ordinary parenchyma ; a tangential sec- 

 tion, however, shows that the cells are elongated, somewhat 



