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THE MYXOSPORIDIA OT? THE BEAUPORT REGION. 227 



ered with a cover glass sealed with paraffin. The spores were not studied until 36 hours after being re- 

 moved from the host, and by that time large numbers of trophozoites had sporulated, although very few 

 spores were present when they were placed on the slide. Spores formed under such conditions are often 

 smaller tlian those produced under more favorable circumstances. 



Ceratomyxa abbreviata, n. sp. (PI. xix, fig. 38-41.) 



Trophozoite. — Vegetative trophozoites colorless, elongate, pyriform, with usually a very long, 

 slender posterior process (fig. 38, 39). Progressive movements rapid. Ectoplasm abundant, very dis- 

 tinct from endoplasm , thickened at anterior end , and usually forming a distinct layer over entire tropho- 

 zoite; attenuated posterior process usually composed entirely of ectoplasm, but rarely the endoplasm may 

 extend into it for a short distance; at anterior end tlie ectoplasm forms short, tapering or filiform pseu- 

 dopodia. Endoplasm has a very characteristic longitudinally striate appearance ; a few small fat glo- 

 bules present at anterior end. 



Length of vegetative trophozoites up to about go^, width io-i2m; diameter of rounded sporulating 

 trophozoites about 27^1. 



Disporous. 



Spore. — Very characteristic. Roughly crescent-shaped; diameter of valves exceptionally great in 

 comparison with their length (fig. 41). Baseof valves circular in cross section or slightly compressed par- 

 allel to longitudinal plane (fig. 40) ; ends of valves rounded, slightly unsymmetrical. Sporocyst excep- 

 tionally tough and resistant to reagents. Capsules large, prominent, located at some distance from cap- 

 sular side and opening on opposite sides of spore midway between capsular and postcapsular margins. 

 Sporoplasm distinct, finely granular, confined entirely to one valve. 



Length of spore 17/x, width 14^^; diameter of capsules 4. Sm- 



Habitat. — Found in the gall bladder of a sharp-nosed shark, Scoliodon ierrcB-novcs, taken August 2, 

 1912. Although a number of these sharks have been examined, this is the only time this species has 

 been found. 



•;i^ Ceratomyxa flagellifera, n. sp. (PI. xix, fig. 42, 43.) 



Trophozoite. — Vegetative trophozoites colorless, short, pyriform, tapering rapidly toward the pos- 

 terior end (fig. 43). In some cases the posterior end may be divided into a number of long, slender, trans- 

 parent processes. The anterior end bears large numbers of extremely long, filiform pseudopodia, which 

 can be seen to sweep slowly back like a whiplash until they come to lie by the side of the body, some- 

 times extending some distance beyond the posterior end. Progressive movements slow. Ectoplasm 

 clear, transparent, forming a distinct layer at anterior end, which gives rise to the pseudopodia and often 

 extends for some distance along the sides. Endoplasm has a very peculiar structure ; in large tropho- 

 zoitesfiUed with large numbers of rod-shaped, bacterialike bodies, which usually lie witli their long axes 

 parallel to the long axis of the trophozoite. These bodies are much more abundant in the anterior half 

 of the trophozoite, gradually decreasing in number toward the posterior end, which is very transparent, 

 itslimitsbeing often very difficult to make out. In smaller trophozoites the bacterialike bodies are much 

 less numerous and may be entirely absent, the endoplasm then showing a distinctly vacuolated struc- 

 ture. In the larger trophozoites there can usually be seen one to several rounded bodies composed of 

 clear, transparent, protoplasm containing a few small, refringent granules (fig. 43). 



Length of vegetative trophozoites up to about 115-120^; width about 40-45^- 



Disporous. 



Spore. — Valves greatly elongated, conical, with rounded ends (fig. 42). Sutural line forming a promi- 

 nent ridge around spore. Capsules large , conspicuous, opening on opposite sides of spore at some distance 

 from capsular margin. Coiled filaments very distinct. Sporoplasm distinct, granular, symmetrically 

 situated, but extending only a short distance into each valve. 



Length of spore iiSm, width 12^; diameter of capsules 6m. 



Habitat. — Found in the gall bladder of a shark, Carcharhinus sp?, taken July 27, 1911. 



Remarks. — The rounded protoplasmic bodies which occur in the larger trophozoites are probably 

 gemmules. They appear strikingly like the gemmules of Sinuolinca dimorpha. (See Davis, 1916.) 

 In a number of cases these bodies were seen to escape from the mother trophozoite , breaking through near 

 the posterior end , where the outer covering appears to be much weakened. I have considerable evidence 

 that this method of reproduction is not rare in Ceratomyxa. 



