234 BULLETIN OF THE BUREAU OF FISHERIES. 



^ Sinuolinea opacita, n. sp. (PI. xxiii, fig. iii, 112.) 



Trophosoiie. — Vegetative trophozoites colorless, opaque; rounded or slightly irregular in shape; 

 slowly amoeboid, forming a number of short, lobose pseudopodia (fig. iii). Ectoplasm not distinct, 

 except around ends of pseudopodia, where it forms a thin, hyaline layer. Endoplasm opaque, finely 

 granular, witli numerous greenish-yellow fat globules varying greatly in size. 



Diameter of rounded sporulating trophozoites about 22ju. Several exceptionally large trophozoites 

 were seen with a diameter of about loo/x. 



Disporous. 



Spore. — Nearly spherical, with flattened, lateral appendages extending from the postcapsular side 

 of each valve (fig. 112). Sutural plane slightly twisted on its axis; sutural line forming a prominent 

 sinuous ridge around spore. Capsules large, opening at some distance from the capsular margin. Coiled 

 filaments distinct. Capsulogenous cells large and easily distinguishable. Sporoplasm distinct, finely 

 granular, containing several comparatively large fat globules. 



Diameter of spores about 12-13^, diameter of capsules 4/1. 



Habitat. — Occurs in the urinary bladder of Paralichthys albiguttus. Found in only one fish taken 

 August 4, 1914. Both vegetative and sporulating trophozoites were abundant. 



Sinuolinea brachiophora, n. sp. (PI. xxm, fig. 113.) 



Trophozoite. — Colorless; rounded to somewhat irregular in shape. Ectoplasm distinct, hyaline. 

 Endoplasm granular, with numerous large fat globules. Spores arranged in the trophozoites with their 

 postcapsular sides in contact and tlie armlike processes mutually interlocking. • 



Disporous. 



Spore. — Presents a remarkable appearance. Central portion approximately spherical, with long, 

 lateral, armlike appendages extending from each valve (fig. 113'). These appendages are empty except 

 at extreme distal ends, which contains a granular mass, probably the remains of the parietal cell. 

 Sutural plane slightly oblique to longitudinal axis, not distinctly twisted on its axis, sutural line forming 

 a prominent ridge around spore. Capsules large, conspicuous, opening at some distance from capsular 

 margin; capsulogenous cells exceptionally large and distinct, the nuclei being plainly visible by the 

 sides of the capsules. Capsulogenous cells and capsules occupying more than one-half cavity of 

 sporocyst. Sporoplasm distinct, finely granular. 



Length of spore exclusive of appendages 9-11/1, length of appendages i8-22;ii, width of spore about 

 9;:, diameter of capsules 3. 5m. 



Habitat. — Lives in the ininary bladder of Paralichthys albiguttus. Found only in one fish taken 

 August 13, 1914. Vegetative trophozoites were abundant, but only a few spores were found. 



Remarks. — Possibly this species should be made the type of a new genus, but the spore undoubtedly 

 more closely resembles that of Sinuolinea than any other genus. In many respects this species is very 

 similar to S. opacita, which occurs in the same host. 



Family MYXTDITD^ Thelohan. 

 Genus Msrxidium Butschli. 

 Myxidium incurvatum Thelohan. (PI. xxni, fig. 114-118; pi. xxiv, fig. 119-121.) 



Myxidium incurvatum Thelohan, 1895, p. 341. 



Trophozoite. — Vegetative trophozoites colorless, translucent, rounded to somewhat irregular in 

 shape, with usually a single short, lobose pseudopodium of ectoplasm, which may extrude short conical 

 or filiform pseudopodia along its margin (fig. 114, 115). Occasionally the pseudopodium may be drawn 

 out into a long, slender process (fig. 117). In some cases after being on the slide for some time the 

 trophozoites contract into a rounded mass covered with large numbers of short, hairlike pseudopodia. 

 Ectoplasm clear, transparent, distinguishable only in the pseudopodia. Endoplasm distinctly, some- 

 times coarsely, granular, with a few comparatively large, greenish-yellow fat globules; usually several 

 rounded, denser sporoblast cells can be distinctly seen. After having been on the slide for some time 

 the endoplasm maybe fairly riddled with large, clear vacuoles (fig. 117). Thesurfaceof the trophozoites 

 is distinctly viscid, and it is common to find several individuals clinging closely together in an irregular 

 mass, in which it is impossible to distinguish the outlines of individual trophozoites. 



Diameter of rounded disporous trophozoites about 13-15^, of monosporous about lo-ii^. 



Monosporous and disporous, the majority being disporous. 



