THE MYXOSPORIDIA OF THE BEAUFORT REGION. 235 



Spore. — Cylindrical, valves rounded at ends (fig. 119-121). Capsules large, opening on each side of 

 spore at right angles to the longitudinal plane. Sutural line can be faintly seen extending around 

 spore just inside the capsular pores. Filaments when extruded in HCl remained tightly coiled 



Length of spore 8-9M, width 5-6m; diameter of capsules about 3fi. 



Habitat. — Common in the gall bladder of Fundulus majalis, Gambusia affinis, and Mugil cephalus. 

 Found in 5 out of 16 adult F. majalis taken July 9 and 10. 



Remarks. — Although the shape of the spore differs somewhat from Thelohan's figure, tlie difference is 

 hardly sufficient to justify considering it specifically distinct. This species has a remarkably wide 

 range of hosts. Thelohan has reported it from the gall bladder of Nerophis cequoreus, Sygnathus acus, 

 Callionymus lyra, Bleniiius pholis, Trachinus draco, and Scorpcena scrofa. 



'''^' Myxidium glutinostun, n. sp. (PI. xxiv, fig. 122-124.) 



Trophozoite. — Vegetative trophozoites colorless, elongated or somewhat irregular in shape; slowly 

 amoeboid, moving by means of abroad, lobosepseudopodium of hyaline ectoplasm (fig. 122). Ectoplasm 

 not distinct except in pseudopodium. Endoplasm finely granular, with a number of rounded homo- 

 geneous spherules, which vary greatly in size and can be only indistinctly seen. The mature spores 

 while still within the mother trophozoites are surrounded by a clear, refractive gelatinous envelope. 

 This envelope only appears as the spore matures, and its absence around one spore in figure 123 is prob- 

 ably due to the fact that one spore matures a little before the other. A similar difference in the rate of 

 development of the spores has been observed in other species. 



Diameter of rounded sporulating trophozoites about 20/1. 



Disporous. 



Spore. — Cylindrical, ends of valves rounded except at one side, where the capsules open at the apex 

 of a small, conical elevation (fig. 124). Spore characterized by the presence of a transparent, homo- 

 geneous gelatinous envelope, which under the microscope has the appearance of a bright ring or halo. 

 This envelope is distinctly viscid, and small particles can often be seen adhering to it. Capsule large, 

 distinctly pyriform, opening on each side nearly at right angles to the longitudinal plane. 



Length of spore lo-iifi, width 6^; diameter of capsules 3m- 



Habitat. — Not uncommon in the gall bladder of Cynoscion regalis. 



i- Myxidliun phyllium, n. sp. (PI. xxiv, fig. 125-127.) 



Trophozoite. — Exceptionally large; flattened, leaflike, usually folded on itself; motionless, no signs 

 of pseudopodia observed (fig. 125). Ectoplasm forming a distinct transparent layer around entire 

 trophozoite. After being on slide for some time ectoplasm usually becomes covered with very numerous, 

 short, indistinct, hairlike processes, which are not present when first removed from the host. Endoplasm 

 finely granular, with numerous fat globules, not vacuolated. 



Diameter of trophozoites up to 1.35 mm. 



Polysporous. 



Spore. — Fusiform, slightly truncated at each end where capsules open (fig. 126). Sporocyst with 

 numerous longitudinal ridges. Capsules large, opening at ends of spore. Sporoplasm finely granulai', 

 with several small fat globules. 



Length of spore i im, width S/i ; diameter of capsules 3/1. 



Habitat. — Occurs in the gall bladder of Gambusia affinis. Only one trophozoite was found in each 

 fish. A small percentage only of fish were infected, and in every case the infected fish were partly 

 grown individuals about 2.5 cm. long. 



Remarks. — This species has been found only in fish collected in one locality and was the only species 

 found in these fish. Numbers of Gambusia from other localities have been examined and a considerable 

 percentage found to be infected with M. incurvatum but never with phyllium. 



Genus Sphaeromyxa Thelohan. 

 Sphseromyxa balbianii Thelohan. (PI. xxiv, fig. 128-131.) 



.SphtBTomyxa balhianii, Thelohan, iSgs, p. 342. 

 SphtBTomyia balbianii, Gurley, 1894, p. 282. 



Trophozoite. — Small vegetative trophozoites colorless; slowly amoeboid; surrounded by a homo- 

 geneous, hyaline layer of ectoplasm (fig. 128) from which arise short, lobose pseudopodia. In some 

 instances when moving in the same direction for some time the ectoplasm may accumulate at the (tern- 



