814 PROCEEDINGS OF THE THIRD ENTOMOLOGICAL MEETING 



In the Table on next page I have given the results of my examina- 

 tion of the gut only according to Hutchinson's method and then the 

 same gut is crushed with the body of the moth and the juice is ex- 

 amined according to the Pasteur method to ^ee in which system more 

 pebrinized moths are detected. The eggs were from diseased stocks. 



Thus out of 844 moths the gut only of which was first examined and 

 the same gut was crushed with the body of the same moth and 

 examined separately 333 and 334 pebrinized moths respectively were 

 detected. 



In about 2 per cent, of the pebrinized moths I have seen more pebrine 

 spores in the body excluding the gut but very few in the gut. In about 

 13 per cent, of the moths more pebrine corpuscles were seen in the gut 

 than when the whole body including the gut was crushed. In about 

 85 per cent, the proportion of pebrine was equal. The corpuscles were 

 distributed in the body- juice when the body including the gut was 

 crushed and there was no difiiculty in detecting them. In very few 

 cases one or two pebrine corpuscles were detected in the gut but not 

 a single one in the body- juice. In rare cases I have noticed one or two- 

 corpuscles in the body-juice but not a single one in the gut. I had 

 to throw away rather more than 5 per cent, of the moths as their gut& 

 could not be distinguished on account of decomposition of the moths. 

 I have also noticed that if the entire gut is not crushed well and a minute 

 part of it is taken by a needle and examined as advised by Mr. Hutchinson 

 pebrine spores may escape notice when the infection is very mild ; if 

 it is crushed thoroughly it is possible to detect a few Spores in about 

 two or three per cent, of the moths where the Pasteur method may fail 

 to detect them if the moths are not treated as stated later on. {Vide 

 Bulletin No. 74 also, written by me.) 



It has been noticed that when the moths are infected with pebrine 

 acutely or moderately there is no difiiculty in detecting pebrine cor- 

 puscles by either of the methods ; but when the mfection is very mild 

 the detection becomes difiicult by both the methods and in a few cases it 

 takes more time if examined according to the Pasteur method. This 

 difiiculty is obviated if the mother-moths are kept at about 70°-90°F. 

 for about five or six days after oviposition. The contents of the colon 

 are dried up if the moths are kept at about 160° to 180°F. for about 

 three or four hours before examination. The moths in the above table 

 could not be dried well as it would have been difiicult to separate the 

 gut from the body. I have noticed that the multiplication of pebrine 

 corpuscles is more rapid from l\Iay to October than from November to 

 April. In summer they propagate quickly but their multiplication is 



