14 



I'KALTIC'AL HAt'TEKlOUXlY. 



DKMONSTRATTON VI. 



Staininf.' of Flaj^ella — Cultures of Bacillus Authracis 



Flagella Staining. 



Ordinary methods of staininjf do not suffico to 

 diMnouHtratc Haj^^ella. so that HptHual staining procosHt^s 

 aro rcHiuirod. KmulHioiiH in storilediHtillod wator of 18 

 Ijour a^ar cultures of Bacillus typliosus ami of Spirillum 

 cholorao asiaticac arc furnished. 



1. Muir's modification of Pitfield's method. 



The following re-a^ents are re([uired: 



The mordant, 



10% ai^ueous solution tannic acid, filtered 10 cc. 

 Saturated acjueous solution, bichloride 

 of mercury ...... 



Saturated aqueous solution, alum . 

 Ziehl's carbol-fuchsin .... 



This mordant will keep for one or two weeks. 



The stain. 



Saturated aqueous solution of alum, 



filtered ....... 



Saturated alcoholic solution of gentian 



violet ....... 



This stain will keep two or three days. 



The cover glasses used must be thoroughly cleansed. 

 This may be easily effected by rubbing first with a clean 

 cloth, then washing in a mixture of equal parts of 



5 cc. 

 5 cc. 

 2 cc. 



10 cc. 



2cc. 



