FLAGKLLA STAINING. 



15 



alcoliol and ctlu^r, and wliili^ moist passing tluMii tlirouj^li 

 tho Hamo. Covor ^lasst's may also be cU^aiiscd by tlio 

 motliod advised by van Krmcn^licm, as follows: 



Boil the covor glasses for Hvo minutes in tlu^ follow- 

 ing solution, 



Potassium bicliromate . . 6 grammes. 

 Sulphuric acid . , . . (> grammes. 

 Water KM) cc. 



From this solution wash throuj^h several dishes of 

 distilled wati^r, and then place in alcohol. Before using 

 pass through the flame. 



i^fi) Prepare thin films from tht^ emulsions; dry and 

 fix in the Ha me. 



(6) Cover the film (h<>ld in forceps) with the mordant, 

 and hold high over the flame till it steams gently for 

 one minute. (Or float the film in the warmed mf)rilant 

 for the same period.) 



(r) Wash thoroughly in large amounts of distilled 

 water. 



((/) Pour on now some of the stain, and again heat 

 gently for one minute (or place in the warmed stain). 



{(') Wash in water, dry and mount in Canada balsam. 



Those bacteria which stain by Gram's method can 

 be treated after the stain by iodine in the usual manner. 

 Fair results are usually obtained by this metiii/ I. 

 Better microscopic pictures can bo secured by van 

 Ermorighem's method, but his method is longer and 

 usually requires considerable practice. 



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