16 



PRACTICAL BACTERIOLOGY. 



Cultures of Bacillus Anthracis. 

 From the culture tube supplied inoculate, 

 {(i) an agar-agar (slope) tube; 

 (h) a potato tube; 

 (c) a gelatine (stab) tube; 

 ((I) a broth tube; 



(^0 a broth tube, to which carbolic acid has been 

 added in proportion of 1 to 1000; 

 (/) a litmus milk tube. 



Place all except (c) in the incubator at ST-'C and 

 keep (c) at 20"C. ' 



Examine these growths at the end of 24 48 and 72 

 hours, noting the characters of growth on the various 

 media. 



