w 



ANTHRAX SEPTICAEMIA. 



m 



spread out the drop into a thin film, and carefully slide 

 the two cover glasses apart. 



{(f) Open carefully the thorax, cutting througli the 

 costal cartilages, and expose the heart. 



{h) Sterilize a portion of the exposed heart wall by 

 searing it with a heated glass rod. Push through this 

 part, a sterile pipette and withdraw the blood of the 

 chambers. Several pipettes may be filled in this man- 

 ner, from one of which cover glass preparations can be 

 made as described in (/) for staining. On withdrawing 

 pipettes fuse the open ends carefully in the flame. 

 Preserve the pipettes for the making of plate cultiva- 

 tions. 



The remaining viscera of the animal may be removed 

 and preserved in 4% formalin for the after study of 

 sections. 



Staining of the Cover Glass Films. 



Fix the films after drying by passing through the 

 flame. Better results are secured in staining the pre- 

 parations from the spleen pulp and the blood by fixing 

 the films in a 10% solution of formalin in 90% alcohol, 

 for one minute. 



2. Staining with eosin and methylene blue. 



(a) Place the fixed films in eosin ^ to 1 minute. 

 (6) Wash in water and then dry. 



(c) Place now in Loeffler's methylene blue for 1 to 

 2 minutes. 



{d) Wash in water, dry and mount in Canada balsam. 



,1 



If 



I- III 



i 



