30 



PRACTICAL BACTERIOLOGY. 



(/) Cover the section with eosin for 1 minute, and 

 then wash off with water. 



((/) Remove the water with blotting paper, and treat 

 with the anilin oil — xylol mixture, till no more blue stain 

 comes away. 



(h) Wash several times in xylol and mount in Can- 

 ada balsam. 



The section might be previously stained in picro 

 carmine, and then put through this process, with 

 the exception of the eosin staining. Either method 

 usually shows both the fibrin net work andPneumoccocci 

 well, when examined with the ^^ oil immersion lens. 



Staining of Tissues for Pyogenic Micrococci. 



6. Stain sections of erysipelas of rabbit's ear. 



(a) By Gram's method, counterstaining in eosin 

 (page 18). 



(6) By "* ram's method, previously staining in picro- 

 carmine (page 13). 



7. Stain the sections of kidney, from acute suppura- 

 tive nephritis. (Staphyloocus pyogenes aureus). 



(a) With eosin and methylene blue (page 21). 



(h) By Gram's method, previously staining with 

 picrocarmine or counterstaining in eosin. 



These methods may be employed for other tissues, 

 containing the pyogenic micro-organisms. 



