36 



PRACTICAL BACTERIOLOGY. 



1: 



I 



■; i' 



f- 



blotting paper. Heat the section carefully to melt the 

 paraffin, rinse through xylol to free the section from 

 the paraffin, then in alcohol to free from xylol. From 

 the alcohol place directly into the carbol-fuchsin. 



2. Staining in sudan III solution. 



(a) Place the sections for 10 minutes in the sudan 

 III stain. 



(h) Wash for 5 minutes in 70% alcohol. 



(c) Rinse in water and transfer for 1 minute to 

 LeoflFler's methylene blue. 



(d) Rinse in water, dehydrate in absolute alcohol, 

 clear in xylol and mount in Canada balsam. 



Leprosy Tissues, 



Bacillus leprae stains like the Tubercle bacillus, but 

 more readily, and is more rapidly decolorized. It does 

 not stain with sudan III. 



3. Stain sections by the following methods: 



(a) Carbol-fuchsin for 20 to 30 minutes, treating 

 afterwards as for tuberculous tissues. 



(b) Weigert's method and eosin. 



The Leprosy bacilli are always present in large 

 numbers in the leprous nodules, and the discharges in 

 ulcerating tubercular leprosy. 



Cultures of Tubercle Bacilli from Guinea Pig. 



The guinea pig has been inoculated three or four 

 weeks previously with sputum from pulmonary Tuber- 

 culosis, and has been killed by chloroforming. 



