TUBERCLE BACILLI FROM GUINEA PIG. 



37 



The animal is opened in the usual manner, and the 

 enlarged inguinal and iliac glands are removed with a 

 sterile forceps, and broken up in sterile capsules. 



4. From the pulp in the capsules inoculate freely, 

 (a) 6 blood serum tubes; 



(6) 6 glycerine broth tubes; 



(c) 6 glycerine agar tubes. 



After inoculation seal the tubes with ordinary wax 

 or paraffin, so as to prevent evaporation. 



Incubate at 37 C. and examine at the end of 7, 10, 

 14, and 21 days. 



5. From the culture of Bacillus diphtherise furnished 

 inoculate, 



(a) a blood serum tube; 

 (6) a glycerine agar tube (slope); 

 (c) a broth tube. 



Place in the incubator at 37^0. and examine at end 

 of 18, 24 and 48 hours. 



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