BACILLUS TYPHOSUS. 



43 I 



4. Make inoculatioiiH of Bacillus typhosus and Ba- | 

 cillus coli communis on, j 



(a) agar-agar tubes; 



(/>) gelatine (slope) tubes; . | 



(c) liquefied grape sugar gelatine; | , 



{(I) potato tubes; 

 (e) peptone tubes; 



(/) litmus milk tubes. | 



Incubate all except the gelatine tubes at 37 C. Ex- | ; 



amine and contrast the growths from day to day. 



5. Sections of mesenteric gland, spleen and liver 



from Typhoid fever cadaver. i 



In the tissues Bacillus typhosus is found scattered \ 



in clumps, so that a number of sections may have to be i : 



examined before finding any bacilli. At least six sec- | 



tions of each of the tissues should be stained. I 



{(() Stain in Loeffler's methylene blue, leaving in the 

 stain from 30 minutes to 2 hours. Rinse in water | 



and then in .1% acetic acid for 2 or 3 minutes; dehy- '^ 



drate in absolute alcohol, clear in xylol and mount in 

 balsam. 



(fe) Stain in Stii'ing's gentian violet solution. 



Gentian violet .... 5 grammes. 



Alcohol 10 cc. ' 



Anilin oil ..... 2 cc. 



Water H8 cc. 



Leave sections in this stain for 10 minutes. Rinse 

 in water and then in .1% acetic acid for 2 or 3 minutes; 

 dehydrate in alcohol, clear first with oil of cloves and 

 then wash several times with xylol before mounting in 

 balsam. 



