50 



PRACTICAL BACTERIOLOGY. 





if 



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Class Demonstration. 



(a) Inoculate a guinea pig subcutaneously with .25 

 cc. of a 72 hour broth culture of Bacillus tetani, tearing 

 the tissues slightly at point of inoculation. 



(?)) Inoculate a guinea pig subci-taneously with .5 cc. 

 of a broth culture of Bacillus oedematis maligni. 



(r) Inoculate .5 cc. of a broth culture of Bacillus 

 capsulatus aerogenes into the posterior auricular vein 

 of a rabbit. Five minutes after the inoculation, kill the 

 rabbit by a sharp blow on the back of the neck. Keep 

 the body in a room at a temperature of 20"C and exam- 

 ine after 18 to 24 hours. Gas formation is seen in the 

 veins everywhere, the viscera will be " foaming," and 

 the animal generally emphysematous. 



