BACILLUS TETANL 



51 



DEMONSTRATION XX. 



Bacillus Tetani — Bacillus (Edematis Maligni— Bacillus Capsulatus 



Aerogenes — Examination of Animals Inoculated with Bacillus 



(Edematis Maligni and Bacillus Capsulatus Aerogenes. 



1. Make hanging drop preparations from the broth 

 culture of Bacillus tetani. 



Slight motility can usually be detected. 



2. Make cover glass films from the broth cultures of 

 Bacillus tetani, Bacillus oedematis maligni and Bacillus 

 capsulatus aerogenes. 



After clearing in 10% acetic acid, stain in anilin 

 gentian violet. Note the presence and position of the 

 spores in the two former bacilli. 



3. Examine carefully the guinea pig inoculated with 

 Bacillus cedematis maligni, noting the .extent and char- 

 acters of the cedema. Reflect the skin in the usual 

 manner. Make cover glass smears from the oedema 

 fluid. 



Open up the abdomen, and removing spleen, make 

 cover glass preparations of the splenic pulp, as de- 

 scribed under anthrax. In like manner make cover 

 glass preparations from the heart's blood. 



After drying fix these cover glass films in the form- 

 alin fluid for 1 minute. 



Stain the films with eosin and methylene blue. 



