74 CLINICAL MICROSCOPY AND DIAGNOSIS. 



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SECTION II. 

 Blood. 



Blood examinations require the use of special instru- 

 ments and reagents. The instruments which will be 

 needed besides a microscope, are a haemoglobinometer 

 (Fleischl's), a haemacytometer (Thoma's) and a spear 

 headed needle for obtaining blood. A haematocrit and 

 centrifuge are very useful when much blood work has 

 to be undertaken. 



The following reagents will be required: 



Toison's dilution fluid, 



Glycerine 30 parts 



Sodium sulphate . . . . 8 " 



Sodium chloride .... 1 " 



Methyl violet 025 " 



Water 160 " 



Benzol and chloroform, and a mixture of these two 

 having a specific gravity of 1055. 



Ehrlich's triple stain. 



Saturated aqueous solution acid fuchsin 2| parts 



Distilled water 2 " 



Saturated aqueous solution orange G. 6 " 

 " " " methylene green 6J " 

 Add the last slowly, drop by drop, constantly stir- 

 ring. 

 Absolute alcohol .... 5 parts 

 Distilled water 10 " 



