chemical analysis and bioassay of pesticide chemicals in fishes, and 

 the plants and animals on which they depend; definition of the immediate 

 and cumulative effects of the chemicals; and the determination of the 

 toxic and lethal levels of both dissolved and ingested pesticides. 

 Standards for pesticide content of the environment which can be endured 

 without severe chronic effects are one objective of these studies. 

 Tolerance limits of various organisms are another . 



Oil Pollution - Research is needed on the effects of oil on sport 

 fish species, their foods, and upon waterfowl. The Bureau assists in 

 determining areas of pollution and in the formulation of regulations 

 covering dumping of oils in prohibited zones. 



Estuarine Studies - The Sandy Hook Marine Laboratory (New Jersey) 

 commenced a study in FI-62 of the Shrewsbury River estuary. Plans are 

 to expand this study to include areas on both the Atlantic and Pacific 

 Coasts, and to include documentation of environmental conditions 

 affecting young game fish and their foods. 



Experimental Biology - A prerequisite to determination of the effects 

 of pollutants is an understanding of the survival, growth and well- 

 being of marine organisms in relation to their environment. In FY-62, 

 an experimental laboratory program was commenced at the Bureau's 

 Sandy Hook Laboratory to study the influence of various environmental 

 factors including microorganisms, under controlled conditions, on 

 growth and physiology of marine fishes. This involves the establishment 

 of a number of plankton cultures, a determination of their nutritional 

 and physiological requirements, an evaluation of biochemical inter- 

 relationships among plankton species, and test feeding of larval and 

 juvenile fishes with various cultures of plankton tagged with radio- 

 isotopes. This program will be continued in FY-63. 



Geological Survey FY-62 $15,000 



FY-63 $20,000 

 Modifications of the oceans resulting from the activities of man are 

 also the subject of Geological Survey research now in progress and 

 planned for FY-63 - Such research includes the effects and fate of 

 radio nuclides and other pollutants delivered to the oceans by runoff 

 from the land; the possible results of the use of nuclear energy for 

 coastline modification (Project CHARIOT); the effects of man-made 

 structures on coastal erosion; and the effect on ground-water salinity 

 of high-water withdrawal rates in coastal areas. 



DEPARTMENT OF HEALTH, EDUCATION & WELFARE 



Public Health Service FY-62 $1,150,000 



FY-63 $1,500,000 

 Fundamental to the safe disposal of wastes in the marine environment 

 is the full understanding of the effects of such wastes on marine 



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