Chromosomal studies of fish eggs and embryos (Dr. Longwell, NMFS, Mil- 

 ford) will be extended to examine fish species in the Georges Bank-Nantucket 

 Shoals area. Genetically based egg mortality occurring in populations fishes 

 will be assessed and the relative importance of hydrocarbons and natural 

 environmental factors inducing lethal chromosome errors in developing fish 

 eggs will be investigated. 



Specimens of tissue from selected fish and shellfish species from clean 

 and impacted areas will be analyzed (Rosenfield, NMFS, Oxford) for evidence 

 of abnormalities, tissue, and cellular diseases. Also, incidence of anatomic 

 lesions in fish and shellfish from clean and contaminated areas will be 

 monitored. 



Analyses of tissue samples (Gould and Thurberg, NMFS, Milford) from fish 

 and shellfish (e.g., ocean scallops and horse mussels) will continue to 

 identify indicators of the physiological and biochemical health of the organ- 

 ism (such as hematology and respiration rates, shifts in metabolic pathways, 

 induction or suppression of enzyes. 



Hydrocarbon analyses on selected fish and shellfish (adults, juveniles 

 and embryos) will continue at the NOAA National Analytical Facility in 

 Seattle, Washington. 



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