Water Sampling and Storage Procedure 



Samples were obtained from a rosette sampling system consisting of 30- 

 liter G/0 bottles, a temperature sensor, and a transmissometer. Details can 

 be obtained from Dave Folger, USCG, Woods Hole, or John Milliman, Geology and 

 Geophysics Department, WHOI. 



Approximately 3-liter water samples were drawn from the 30-liter G/O 

 bottles directly into a brown glass 1-gallon bottle. These bottles had 

 previously contained glass distilled petroleum ether, n-hexane, or nanograde 

 methylene chloride. After the samples had been drawn from the G/O bottle on 

 the fantail of the OceanuSj the 1-gallon bottles were carried into the main 

 laboratory. Within 30 minutes, 100 milliliters of nanograde methylene 

 chloride were added to the sample, and the sample shaken by hand in a hori- 

 zontal position for 1 minute as determined by the main laboratory clock. The 

 samples were then allowed to sit for 20 to 60 minutes. The water was then 

 emptied via a 500-milliliter graduated cylinder, which was used to measure the 

 water volume. The water-CH2Cl2 interface and methylene chloride were emptied 

 into either 16- or 32-ounce jars. The bottle was rinsed with 20 to 30 milli- 

 liters of CH2CI2 and the rinse added to the sample extract in the 16- or 32- 

 ounce jar. The CH2CI2 extract plus water interface was capped with foil 

 between the cap and the glass jar and sample extract. 



Several of the water samples were saved for extraction efficiency testing 

 with the (approximately) 3 liters of water and 100 millilters of CH CI2 in the 

 1-gallon brown jug. The samples and sample extracts were stored at room 

 temperature. 



Several blanks were obtained during the two cruises. These consisted of 

 adding 100 milliliters of CH2CI2 to 1-gallon empty bottles and shaking for 1 

 minute, pouring off CH2CI2 of 16- or 30-ounce jars, and then rinsing the 

 bottle with 20 to 30 milliliters of CH2CI2. 



A sample of deck washings from R/V Oceanus was taken by running seawater 

 over the deck and collecting this water as it ran off over the side. This 

 sample of about 2.5 liters was poured from a stainless steel bucket into a 1- 

 gallon brown bottle, and 100 millilters of CH2C1„ were added. This sample was 

 deemed necessary due to rain and melted snow runnoff from the deck at several 

 points during cruise 20. 



V-5 



