No trace of oil was found in any part of this survey by any of the three 

 people who participated in various phases of it (M. Jordan, G. Woodwell, J. 

 Hobbie, MBL) . 



Bacteria . Sediment and water samples for bacteria were taken at each 

 sampling site. Samples are being counted by a direct count method using 

 acridine-orange stain and epif luorescent Illumination. In addition, selected 

 samples from each transect were assayed for bacterial metabolism using la- 

 beled amino acids (J. Hobbie, B. Peterson). 



Benthic microalgae . At each site, samples of surface sediments were 

 taken for determination of chlorophyll content as a measure of microalgae 

 (primarily diatom) biomass. These analyses are being done now. Samples were 

 preserved with formaldehyde for taxonomic enumeration of diatons (J. Hobbie, 

 B. Peterson) . 



Benthic macrofauna . Samples were collected along transects at each 

 site. The sampling quadrants were measured to correspond in size and depth 

 to samples taken with the Van Veen grab in the subtidal. Samples preserved 

 with formalde hyde. These samples were taken by Howard Sanders and his 

 colleagues and will be sorted in a preliminary analysis to appraise the 

 sampling technique. If oil comes ashore, these samples will be available for 

 background data (H. Sanders) . 



Benthic meiofauna (small invertebrates of interstitial waters in coarse 

 sands) . Samples were taken at several levels at the two beach sites and at 

 several additional sites by W. Patricia Morse and her students. These sam- 

 ples are being analyzed (P. Morse). 



The strand line . Organic debris on the strand line was collected ran- 

 domly and preserved. A more extensive collection of the full range of macro- 

 algae that could be found along a mile of beach was also made Samples were 

 preserved for later analysis (M. Jordan) . 



Photographers . The sampling program was docximented estensively by two 

 photographers. A set of "standard" photographs was taken at each transect 

 and at each station looking in the cardinal directions. 



Description of Transects 



South Shore - Southwest of the town of Siasconset and immediately east 

 of the USCG Loran Station. The base point of the transect is at the edge of 

 the permanent dune, approximately 2 meters higher than the high tide. The 

 transect runs due south* from this base point. The base point is about 20 

 meters west of a small sand road that runs perpendicular to the beach. A 

 small antenna 73.8 meters north forms a range with the chimney of a house on 



*A11 directions of the compass given in this report are magnetic and have 

 not been corrected to true north. 



V-42 



