26 
sediment sample. The surface samples were taken with sterile 
bottles as were the dye patch samples. The subsurface samples 
were collected with sterile bacteriological samplers (Fig. 2). 
Water for various chemical determinations was collected at most 
profile stations; the hydrological and bacteriological casts 
being interspersed. In all instances, the appropriate dilutions 
were prepared and the lactose tubes were inoculated and incubated 
within an hour of obtaining the water samples. 
Sediment Sampling 
Sediment samples were taken with either a corer or a snapper; 
the latter being used most frequently because the coarse texture 
of the sediments near the outfalls made coring difficult or 
impossible. Immediately after retrieving the sample, a measured 
portion was suspended fn sterile diluent and coliform counts 
were made on the resulting suspensions. An effort was made to 
sample only the surface layer of the sediment. 
Laboratory Procedures 
Determinations of Most Probable Numbers (MPN) of Coliforms 
Standard methods were followed in all details for the 
determination of MPN. Lactose broth and E.M.B. agar respectively 
were used as the presumptive and confirming media. In general, 
all positive presumptive tubes in the three highest dilutions 
showing positives were confirmed. All negative confirmed tests 
were completed. Six dilutions to 107? were prepared from almost 
all of the water samples examined and consequently most tests 
were determinate. Four dilutions, 1071 to 10-4, were used for 
