1898] MICROSCOPICAL JOURNAL. 29 



Leuwenhoeck. — Two volumes of his works, bound in 

 half morocco, nice and perfect for sale at $15.00.-0. W. S. 



SCIENCE-GOSSIP. 



Chlorosis and anaemia. — Dr.Klots having" treated these 

 diseases with nucleo-albumins and bone-marrow, photo- 

 microg-raphed the blood before and after with remarkable 

 results. In four weeks the hsemog-lobin increased as 

 shown by the following- per cents : 54, 57, 64, 70, 74. The 

 increase in weig-ht of the body was from 117 to 119, 121, 

 123, 124 pounds. The number of red blood cells (in mil- 

 lions) was 2.7, 3.0, 3.6, 4.0, 4.1, — an increase of over 50 per 

 cent. 



Carcinoma. — Dr. Palmer Findley of Chicago, says that 

 diag-nosis of cancer of the cervix is often impossible with- 

 out the aid of the microscope. To await development is 

 hazardous. If doubt exists a microscopic examination of a 

 piece is imperative. If pieces cannot be cut, resort to 

 scraping-. Practical knowledg-e of microscopy is essential. 

 This may indicate or avert hysterectomy according as 

 there is a malignant g-rowth or merely an infiamatory lesion. 

 Embed the cutting-s in celloidin preparatory to mounting-. 



Celloidin Embedding. — Cleanse the tissues in cold 

 water. Keep in 4 per cent formalin 12 hours, in 50 per 

 cent alcohol 24 h., in 70 per cent alcohol 24 h., in 95 per cent 

 24 h., in absolute alcohol 24 h. If small, that process may 

 be shortened, the object being- merely to harden them. 

 Then put in dilute celloidin 24 h., in thick celloidin solution 

 24 h., mount on cork for cutting-. After exposure to open 

 air for a few minutes, immerse in 70 per cent alcohol for a 

 few hours, then cut. Double stain the sections with eosin 

 and hematoxylin. For serial sections the paraffin method 

 must be used, but then an oven kept at a proper tempera- 

 ture is a troublesome necessity. 



Freezing. — Animal tissues may be cut in 50-60 minutes 

 if frozen but such preparations are never so satisfactory 

 as those made with celloidin or paraffin. After cutting- 



