1898] MICROSCOPICAL JOURNAL. 5 



tion occurs at length of 2J microns. Stain by Gram's 

 method and by the hydro-alcoholic solutions. 



Fig. 9 shows agar streak culture, 2 days. It is first 

 visible in 24-48 hours as a faint granulation along the 

 inoculating line. Magnifying, small colonies are made 

 out and are colorless, A yellowish white sediment ap- 

 pears in 24 hours. On glucose-agar the growth is larger. 

 In lactose-litmus agar, the colonies appear all through 

 the medium, pink color from surface to bottom. 



Lactose-gelatin with chalk (fig. 10) at three weeks 

 shows larger, opaque colonies, circular and regular in 

 outline. Diameter half a millimeter, 



Grelatin plate colonies : deep, 3 days, and surface, 4 

 days, are shown in figures 11 and 12 enlarged 100 dia. 

 They are quite circular with regular and well-defined 

 margin. Diameter, .25 mm. On potato, there is no 

 growth. 



Cover-glass preparations, xl500, are shown in figures 

 13 and 14, the former a 3-days milk culture and the other 

 a 2-days glucose-bouillon culture. Figure 15 shows 

 growth and segmentation. 



The milk forms firm coherent clots in 20-30 hours or- 

 dinarily, but in incubator in 12 hours. It is strongly acid 

 with faint sour odor. 



Bacterium discissum occurs in spontaneously soured 

 milk. They are oval, in pairs, in chains or singly. Sin- 

 gle forms are half longer than broad — dimensions 1,5 

 microns by 1 micron. In glucose bouillon, the chains are 

 most abundant and larger than elsewhere. The individ- 

 ual elements of the chains are nearly round, segmented 

 and vary in size as shown in figure 20, 



Grlucose-agar streak culture of 10 days growth shows 

 as in figure 16, In glucose bouillon the turbidity appears 

 later but is equally dense. 



Gelatin tube stab culture of 5 days standing, fig. 17 ; 



