1898.] MICROSCOPICAL JOURNAL 221 



the slide, and drying, a confusing mass of needles and 

 crystals >are found mixed up wiih the scattered corpus- 

 cles. This crystalline debris, may be washed away by 

 pure water gently dripped on the slide without disturb- 

 ing the corpuscles. They may then be colored with the 

 eosin and methyl blue solutions in succession, covered 

 with cover glass, and examined with a twelfth oil-immer- 

 sion at leisure. 



The methods suggested may he tried by those accus- 

 tomed to fixing blood with the alcohol-ether solution, or 

 by heat, or by saturated alcohol sublimate solution, and 

 the relative results compared. 



For staining the malarial organism after fixing the 

 blood corpuscles, the method is : 



1. The specimens are stained with eosin (one-half of 

 one per cent eosin in ordinary alcohol) for five to fifteen 

 minutes ; the solution will not stain too deeply. 



2. Wash in running water and dry in air. 



3. Stain with methyl blue (one drachm of the labora- 

 tory solution to an ounce of water is strong enough), the 

 time it takes to count ten — eight to ten seconds is long 

 enough. 



4. Wash in running water, dry, and mount in balsam. 

 The blue stain colors the parasites : the danger is in over- 

 staining with the blue. Grood success is had by using a 

 10 per cent solution of methyl blue in alcohol, staining 

 two or three minutes. But for the crescentic forms of 

 the sestivo-autumnal fevers which stain with more diffi- 

 culty than the ordinary forms of tertain type, the watery 

 solution of blue is necessary. — Ind. Med. Journal. 



For Sale. — A $45 microscope stand for $25. Address: 

 W. A. Murrill, Ithaca, N. Y. 



For Sale. — Fatty Ills and their Masquerades, By 

 Ephraim Cutter, M. D, LL. D., and J. A. Cutter, B. So. 

 M. D. $1.00. Box 494, 120 Broadway, New York. 



