Fics. 1-4.—The four tentative microscopical patterns of coagulation (sche- 
matic). The drawings have been combined from observations, by means of the 
phase-contrast microscope, of about 5,300 samples of hemolymph, in standard 
conditions of preparation of the films between slide and coverglass. 
Fig. 1: Pattern I. Island of coagulation around a hyaline hemocyte (co- 
agulocyte). A granular hemocyte and a macronucleocyte of small size are not 
involved in the process of coagulation. Extension of the coagulum around the 
island and reorganization of the granular clot into meshworks of granular 
fibrils have not been represented in the drawing. (Compare with photomicro- 
graphs in Grégoire and Florkin, 1950, pls. 1 to 10; Grégoire, 1951, figs. 1-3, 
10-12, 14, 27, 28; 1953b, figs. I, 2, 20, and 22; 1955a, figs. I-6, 10, 15, 21, 23; 
27; and in the present paper, pl. 1, figs. 1, 2, 3, 4, 5, and 7.) 
Fig. 2: Pattern IJ, Extrusion of cytoplasmic expansions by hyaline hemo- 
cytes, which appear in the drawing elongated and reduced in size, except three 
corpuscles, bulging out at the periphery. The fan-shaped direction of the 
cytoplasmic filaments from an incidental bubble is induced in part by currents 
in the spreading film of hemolymph. However, this picture has also been 
observed to develop slowly, when the hemolymph was at a standstill. Reaction 
in the plasma in the shape of glassy elastic veils (no cell fibrin) inside of the 
cytoplasmic systems built up by the unstable hemocytes. The veils are fre- 
quently detected only by their stretched folds. By pressure exerted on the 
coverglass, the structures embedded in the veils move backward and forward, 
and preserve their relative positions and distances to each other. The other 
categories of hemocytes, agglutinated at random in strands along the highly 
adhesive cytoplasmic expansions of the fragile hemocytes, are not represented 
on the drawing. (Compare with photomicrographs in Grégoire, 1951, figs. 24-26; 
1955a, figs. 17-19, 22, 26, 38, 39, 41; and in this paper, pl. 1, fig. 6.) 
Fig. 3: Pattern III. Association of patterns I and II in the same film of 
hemolymph: extrusion of cytoplasmic expansions by the hyaline hemocytes, as 
in pattern II. Reaction of the plasma consisting of granular veils and of 
islands of coagulation. The islands appear within the veils as circular areas of 
greater density around the hyaline hemocytes. In this drawing, the reaction 
was initiated by alterations in hyaline hemocytes to contact with a foreign body 
(hatched). For the fan-shaped disposition of the structures, see above, pat- 
tern II. The other categories of hemocytes are not represented on the draw- 
ing. (Compare with photomicrographs in Grégoire, 1955a, figs. 11 and 20; and 
in this paper, pl. 1, fig. 9.) 
Significance of pattern III might be questioned as representing merely a 
subsidiary variation of pattern II. However (see legend, plate 1, fig. 9, 
Zophobas latticollis Kraatz, Tenebrionidae), pattern III depicts actual differ- 
ences appearing consistently in the microscopical picture of coagulation of the 
hemolymph, between groups of insects such as Cetoninae (typical pattern II) 
and Tenebrionidae (typical pattern III). On the other hand, as already pointed 
out (Grégoire, 1951), strong mechanical agencies are apt to give deceptive pic- 
tures of pattern III, by inducing extrusion of cytoplasmic expansions from hyaline 
hemocytes in insects in which these reactions do not develop spontaneously in 
the standard conditions of preparation of the films used for these studies (for 
instance, Orthopteroid complex). 
Fic. 4: Pattern IV. No visible modification detected by means of the phase- 
contrast microscope in the plasma surrounding inert or altered hyaline hemo- 
cytes, similar in their appearance to the hyaline hemocytes playing a selective 
part in the coagulation of the plasma in the other patterns. (Compare with 
photomicrographs in Grégoire, 1951, figs. 29 and 30; 1955a, figs. 12, 13, 14, 
and 16.) 
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