NO. 6 HEMOLYMPH COAGULATION IN INSECTS—GREGOIRE 7 
out extension of the coagulation; I (scattered islands of coagulation 
of various sizes, with moderate coagulation of the fluid in the chan- 
nels) ; I*, I**, I*** (islands around all the hyaline hemocytes, sub- 
stantial and general coagulation. In I***, the films appear to the 
naked eye with a bluish opalescent color). 
MICROSCOPY (PARTICULAR REACTIONS) 
Orthopteroid Complex—Pattern I has been uniformly recorded 
in all the insects of the orthopteroid complex listed above. However, 
intensity in the reaction differed in the various groups: in this respect, 
Blattodea and Gryllidae exhibited the most substantial coagulation. 
These groups were followed, in order of decreasing intensity, by 
Mantodea, Phasmoptera, Tettigoniidae, and Acrididae. 
In the present material, alterations in plasma appearing around 
hemocytes other than the fragile hyaline hemocytes were detected 
around a few macronucleocytes of small size (stem cells) in samples 
from Paroecanthus podagrosus and from Phasma sp. As already 
pointed out (Grégoire, 1951, 1955a) such reactions are exceptional. 
Heteroptera——As shown in the list, absence of visible change in 
the plasma was the predominant picture in all the specimens of the 
present material. The few modifications that might suggest subsidiary 
participation of a pattern other than pattern IV were equivocal. 
In Ghilianella sp., Triatoma dimidiata, Rhiginia sp., Apiomerus 
ochropterus (Reduviidae), Mecisthorhinus marmoratus (Pentatomi- 
dae), dark oval hyaline hemocytes, surrounded by a refractile halo, 
underwent sudden clarification after explosive ejection of cytoplasmic 
substance into the surrounding fluid. These discharges did not bring 
about changes in the consistency of the plasma. Similar alterations 
have been described previously (Grégoire, 1955a) in various insects, 
especially in lepidopteran and dipteran larvae and in dipteran adults. 
In several samples from different families, granular precipitates, 
unrelated to the presence of hemocytes in the vicinity, were found in 
films of hemolymph around air bubbles and along the edge of the 
coverglass. Though the preparations were maintained between the 
observations in petri dishes under high moisture, these modifications 
resulted probably from a slight degree of evaporation and conden- 
sation at the periphery of the films. Pressure exerted on these 
precipitates dispersed granular particles ; this reaction is different from 
that taking place under similar mechanical agencies, in an actual 
coagulum, in which extension of the granular and fibrillar structures 
is followed by elastic contraction without dissociation. 
