NO.6  HEMOLYMPH COAGULATION IN INSECTS——GREGOIRE 27 
Homoptera.—Pattern I, with general solidification of the plasma, 
was consistently observed in Cicadidae, Cicadellidae and Fulgoridae. 
In the last family (Laternaria, Phrictus), coagulation of the hemo- 
lymph was especially substantial; the films of hemolymph were in- 
stantaneously transformed into opalescent bluish clots, embedding all 
the hemocytes (altered fragile hemocytes, numerous small macro- 
nucleocytes, and transitional forms to various types of granular hemo- 
cytes). 
Coleoptera—tThe various groups of Coleoptera listed in the table 
exhibited a great diversity in the reactions of their hemolymph in 
vitro. However, predominance of one of the patterns characterized 
several groups. 
Dark hyaline hemocytes, undergoing clarification after discharge of 
substance (see Heteroptera above, and Grégoire, 1955a, discussion, 
p. 129), were observed in Agra sp. (Carabidae) and in Veturius 
platyrrhinus (Passalidae). 
The reactions detected in Scarabaeidae (especially Melolonthinae, 
Rutelinae, Dynastinae) were essentially identical to those reported 
previously as representative of this family. Upon withdrawal, the 
hemolymph became immediately viscous and ropy. The hyaline 
hemocytes, relatively numerous (64 percent of the total hemogram 
in Lagochile sparsa Ohaus) and of small size, extruded spontaneously 
cytoplasmic expansions, soon embedded, like the other hemocytes, in 
the veil-like reaction developing in the plasma (pl. 1, fig. 6). 
In Zophobas latticollis Kraatz, pattern III, characterizing several 
species of Tenebrionidae, developed with a special clarity : cytoplasmic 
expansions of the hyaline hemocytes and transparent glassy veils 
(pattern II) appeared immediately upon withdrawal of the hemo- 
lymph. The consistency of the veils became granular, while circular 
areas of greater density (islands of coagulation: pattern 1) grew out 
around several hyaline hemocytes already involved in the constitu- 
tion of cytoplasmic systems (pl. 1, fig. 9). 
In the specimens of Sandalidae, the film of hemolymph consisted 
of a substantial syrupy granulum embedding tiny nuclei of altered 
unidentifiable hemocytes. The pattern of coagulation could not be 
safely established in these specimens. 
In Lampyridae, dense suspensions of particles normally present in 
the hemolymph of these insects, as in other groups (Coccinellidae, 
various Chrysomelidae), interfered with the detection of the pattern 
of coagulation. 
Among Cerambycidae, subfamily Prioninae (Stenodontes, Calli- 
