28 SMITHSONIAN MISCELLANEOUS COLLECTIONS VOL. 134 
pogon) exhibited one of the most substantial coagulations recorded 
among all the insects examined in this and in the previous studies. 
Hymenoptera.—Rapid collection of the hemolymph without con- 
tamination with foreign tissues was difficult in small specimens and 
in dry ones. In ants, large numbers of specimens were used, and the 
only samples not discarded were those in which a limpid drop of 
hemolymph could be collected and a rapid spreading out of the films 
performed. 
In view of the scarcity of the species available and the large inter- 
specific and intraspecific variations in the reactions observed in this 
order of insects, the predominant patterns could not be established 
safely for several species and groups. Some patterns actually recorded 
in a part of the samples correspond possibly to incomplete reactions. 
Pattern I was observed in all the samples collected from all the 
females, males, and workers of Paraponera clavata (Formicidae) and 
Chlorion (Sphecidae), and pattern III in the three specimens of 
Mutillidae captured. 
In larvae of Camponotus sericeiventris, numerous hemocytes were 
loaded with refractile inclusions, and no modification of the plasma 
appeared in that material. 
The Hymenoptera listed in the table were characterized by the small 
size of their hemocytes and of the islands of coagulation around the 
hyaline hemocytes, even in the samples in which a substantial coagu- 
lation was recorded. In the latter preparations, a considerable ex- 
tension of the coagulation took place from around the islands of 
coagulation, which appeared in the granular clots as small circular 
areas of greater density, centered by the fragile hyaline hemocytes 
and remaining distinct in the general coagulation of the plasma (pl. 1, 
figs. 3, 4, 5). 
Lepidoptera (larvae).—In the two specimens of lepidopteran lar- 
vae, the reactions of the hemolymph in vitro were identical to those 
described and illustrated elsewhere in a large number of caterpillars 
(Grégoire, 1955, pp. 118-120 and pls. IX and X: pattern II, with 
large individual variations in the completion of the process, fre- 
quently incomplete, as in the two specimens listed in the table). In the 
films of hemolymph, refractile hyaline hemocytes underwent clarifi- 
cation after rupture of the cell boundaries and discharge of substance, 
as illustrated in figures 42-50 of the above-cited paper. 
DISCUSSION 
I. The four patterns used in the present study are an attempt to 
classify the disparities recorded in insects with regard to the micro- 
