14 BOTANICAL GAZETTE, | Jan. 
In rare cases pairs of spores that had not completely separated 
were seen, but evidently mature, as in each one the two nuclei 
were demonstrable (figure 16). Sometimes, in strong and rapidly 
developing tubes which had attained considerable length, the in- 
tine of the pollen-spore seemed to be pulled away from the extine, 
or as though it had fallen in or was pushed in by external pres- 
sure which the more rigid extine resisted, as shown in figures 12 
and 13, and finally became knotted up at the tube end of the spore. 
In conclusion, then, the results intended to be presented in 
this paper are: : 
1. That in Tradescantia Virginica, by using the simplest appli- 
ances, and in a very brief time, the two nuclei of the pollen-spore, 
and their descent into the pollen-tube, can be demonstrated. 
2. That in this species the generative nucleus is a large worm- 
like spindle, and precedes the vegetative nucleus into the pollen- 
tube. 
A New Larval Entomophthora. 
BY J. C. ARTHUR. 
(WITH PLATE It.) 
The clover-leaf weevil, Phytonomus punctatus Fabr., is a com- 
paratively new insect in this country. It was first brought 
to public notice in 1881' as very destructive to clover in Yates 
county, N. Y. It has now extended considerably, being abun- 
dant at Buffalo, and in the adjacent part of Canada, and is also re- 
ported from Indiana. It is supposed to have been introduced 
from Europe, where it is common, but looked upon as innoxious. 
In last of May and first of June of this year, the larvee were 
found in a clover field at Geneva, N. Y., dying in vast numbers 
of some parasitic fungus. Again,in October and November, they 
appeared in the same manner over a large lawn. At the latter 
date as fulla study of the fungus was made as limited time would 
permit. It proves to be an undescribed species of Entomophthora, 
and may be characterized as follows: 
Entomophthora Phytonomi (n. sp.)—Mycelium abundant, branched, non-sep- 
tate, colorless, 9-12 in diameter, on the ventral surface of the insect growing 
out in form of rhizoids to act as holdfasts; hymenium over the whole surface 
except the head, 35-45” deep; conidiophores branched at the base, as thick as 
the mycelium ; spores oblong, colorless, 24-28 long by 7-10u thick. Resting 
spores not seen. 
In the larve of Phytonomus punctatus Fabr. Geneva, N. Y., May—June 
and October—November, 1885. 
1Riley, Amer. Nat., xv, p. 751; Rep. U. 8. *t Agric., 1881-2, p. 172; Lintn rst 
Ann. Rep. Insects of N. Y., p. 252. pe a 2, P. er, Fi 
