302 BOTANICAL GAZETTE. [Nov., 
within the diseased berries retains its vitality during the winter 
months and through the agencies of warmth and moisture of early 
spring and summer the asci and sporidia are produced. 
The germination of these sporidia has never been observed, 
but if by any system of culture they can be made to reproduce 
the Phoma of the Black Rot their real nature will be settled be- 
yond dispute. 
EXPLANATION OF Puate IX. Fig. 1. A fragment of epidermis of a dis- 
eased berry, showing five of the black “ pustules” formed by the development 
of the pycnidia. From four of these slender, contorted, worm-like filaments are 
being extruded ; these are the stylospores held together by a kind of mucilage. 
Fig. 2. A section through a bit of the berry, including a pycnidium (P) 
and a spermagonium (S). At O is the osteolum of the pycnidium through 
which the spores escape at maturity. 
Fig. 3. A section of a portion of a pycnidium, more highly magnified, 
showing the basidia, 
Fig. 4. Three stylospoves germinating. 
5. A section through the perithecium or conceptacle of the ascospo- 
rous form, showing the asci, etc. 
ig. 6. Two separate asci, showing the 8 sporidia in each. 
Fig. 7. Four of the sporidia that have escaped from an ascus. 
Fig. 8. An ascus, enclosing 8 sporidia, found June 2, 1886, in grape (de- 
stroyed in 1885 by “ Black Rot”) kept for a week in moist air. From camera 
lucida sketch made by Erwin F. Smith in the laboratory of the University of 
Michigan. Mr. Smith notes that the “ receptacles containing the asci are num~- 
erous, and the asci themselves abundant.” 
Synopsis of North American Pines, based upon leaf-anatomy. * 
JOHN M. COULTER AND J. N. ROSE. 
8. P. monophylla Torr. & Frem. Section almost circular: 
stomata in 18-26 rows: number of ducts two! (.055-.115 mm.) + 
number of cells in bundle-sheath 30-55 : strengthening cells 10 
fibro-vascular region: leaf 1 to 2 in. long. 
In the Sierra Nevada and mountains of California. : 
The single leaf serves well to distinguish this species. It has been consid- 
ered a single leaf or a connate pair, but its minute structure at once decides 
that it ts but one of the two leaves found in P. edulis, and the notion 
*Continued from page 262. eer 
ed 2 Imann, in Bot. Calif. ii. 124, says that the ducts vary from 2 to 14, but We 
have found but two. Our minetonins uate included the type. 
