228 E. D. EVENS ON MOUNTING ALGAE, MOSSES, ETC. 



by Professor C. J. Chamberlain in bis Methods in Plant Histology 

 (Chicago, 1915), to which anyone interested in this branch of re- 

 search is referred. The results obtained by staining with Magdala 

 red and aniline blue, as he directs, are exceedingly beautiful, and 

 it may perhaps be worth while to point out here that the Magdala 

 red used in the process is apparently identical with eosin. The 

 aniline blue which has been found most satisfactory is the 

 mono-sulphonated water-soluble aniline blue known as alkali blue 

 (B.A.S.F.). I also find paraffin (Burroughs and Wellcome's 

 Parolein), as recommended some years ago by Dr. A. Cole, to 

 be a satisfactory mounting medium in place of Venice turpentine. 



References. 



(1) Nature, 1916, 98, 229, I. Jorgensen. 



(2) Kew Bulletin, 1908, Prof. Trail. 



(3) Nature, 1916, 98, 191, A. B. Rendle. 



(4) J.Q.M.C., 1917, 13, 2nd series, 344, A. B. Rendle. 



(5) See The Microscope, 1901, 519, Carpenter. 



(6) Journ. New York Mic. Soc, 1893, 9, 39, Julien (abstracted 



J.R.M.S., 1893, 566). 



(7) J.Q.M.C., 1915, 12, 2nd series, 527, G. T. Harris. 



(8) J.Q.M.G., 1916, 13, 2nd series, 15, G. T. Harris. 



(9) See a resume by Nieuwland, Bot. Gaz., 1909, 47, 237 



(abstracted J.R.M.S., 1909, 401). 



(10) E.g. see Cross and Cole's Modern Microscopy, 1912, 189. 

 See also : 



Museum Journal, 1914, April, 325. 

 Museum Journal, 1916, October. 

 Bot. Gaz., 24, 206. 



J.R.M.S., abstracts, 1894, 277, Lemaire. 

 Guide to Microscope, Heath, 114. 



[Note. — August 1921. — I have since found that it is advisable 

 to fix very delicate objects, such as Protococcus, for a few 

 minutes by adding to the water containing them one-fifth of 

 its volume of Flemming's weaker solution. This should then 

 be well washed out with weak formalin before proceeding with 

 the zinc acetate-formalin treatment. This causes very little 

 blackening. None of the fixing agents above described are ideal, 

 and all cause shrinkage in Cladophora.] 



Journ. Quekett Microscopical Club, Ser, 2, Vol. XIV., No. 87, November 1921. 



