JOURNAL OF ENTOMOLOGY AND ZOOLOGY 133 



The vesicular appearances or more pronounced reticular ar- 

 rangements are produced by reagents. This is clearly seen when 

 comparison is made with the fresh and probably living ganglion 

 and fibers. The fresh strands in nerves and connectives are straight 

 and parallel, while in certain fixed ganglia the strands are irregular, 

 often massed in such a way as to cling at one place and hang free at 

 another, forming a reticulum, as strands of hair or rope would 

 when wet and partly separated here and there. Of all the single 

 reagents tried osmic acid, both before and after boiling in hot water, 

 gave the least distorted picture of fibers and fibrils. 



5. Tigroid substance was seldom seen in a typical distribution, 

 although blue staining material might be concentrated in one or 

 another part of the cell body. 



(Contribution from the Zoological Laboratory of Po/nona College) 



