306 The Botanical Gazette. [July, 
as if spores were present in a dormant condition. This bean 
was placed ina moist chamber to induce the fresh develop- 
ment of spores. March 7, 1894 (a few days later), the pus- 
tules were considerably larger and the material was examined. 
Fresh spores were present in great numbers and dilution cul- 
tures for the separation of the fungus were started on the 
same day. The room temperature was rather low, and on 
the following day none of the spores had germinated though 
some which were seen had swollen to some extent and re- 
fringent granules were appearing. Culture number one was 
then left in a warmer room and on the following day the 
spores were germinating and their study in this condition was 
then made. The germ tubes are very large, equalling or 
even in some cases exceeding the diameter of the spore. The 
first tubes usually arise near the ends of the spore and are 
generally directed at a greater or less angle from the axial 
line of the spore. The refringent granules are quite numer- 
ous and large so that the protoplasm presents a very coarsely 
granular appearance. In the homogeneous protoplasm vacu- 
growing end of the same to be diverted so that the appear- 
ance of dichotomy is the result. On the very young colony 
plasm become quite large and prominent. Also at the ends 
on the plumose branches very short lateral branches noW 
