1895.] Development of Colletotrichum. 307 
arise, several on one side, and the end of the thread is fre- 
quently curved to the same side. Upon these short branches 
are developed the dark bodies which appear in certain of the 
anthracnoses sometimes called secondary spores. 
On March roth from dilution culture no. 2 small colonies 
of the fungus were transplanted to vetch stems in culture 
tubes for pure cultures. In two days a very minute growth 
appeared at the points of the transplantings as fine radiating 
white threads. In a few days more the spots of the central 
point of growth became black by the darkening of the threads 
while the advancing margin of the weft continued white. On 
the 14th spores were found to be developing in considerable 
numbers. By the 16th areas varying from I to 2™ in length 
on the stems were occupied by the very black and thin stroma 
of the fungus. Except where the threads had reached the 
liquid in the bottom of the culture tube there was no consid- 
erable development of white fungus threads. In the liquid 
however quite a profuse growth took place. From the surface 
of the dark stroma in some cases a scanty growth of whitish 
threads arose for a few millimeters from the surface. The dark 
Stroma itself was roughened by the development of irregular 
tuberculate prominences. 
April 20th pure dilution cultures were started to obtain the 
Spores in different stages for the study of germination and the 
following development stages, more especially that these 
might be recorded in photomicrographs while the organisms 
were zn situ. 
The dilutions were made in Petri dishes. On the follow- 
ing day, the 21st, the cultures were examined and no spores 
were found germinating, though they were present in numbers 
and there was no difficulty experienced in finding them in the 
cultures. 
The cultures were examined again on the 22nd, and while 
the spores did not appear as if they were dead there were 
none germinating. On the 23d a few of the spores were found 
to be germinating. , 
On the 24th at 5 Pp. M. additional cultures were made in 
Van Tieghem cells in the following way. The cells were pre- 
Pared and the cover glasses sterilized by passing several times 
through the flame and then placed under a bell jar to protect 
them from gravitating germs while the culture material was 
being placed on them. In order to have a large number of 
