308 The Botanical Gazette. [July, 
spores in a small space about one-half cc. of liquid agar was 
prepared in culture tubes and these inoculated by transplant- 
ing a considerable quantity of the mycelium and attached 
spores from the culture on vetch stems. This would probably 
assure a large number of spores in the liquid. Witha looped 
sterilized platinum needle a small quantity of the inoculated 
liquid was lifted from the tube and allowed to spread upon the 
center of the cover glass, only one transfer being made, and 
the liquid thus was held in a thin layer until solidification took 
place. Many of the spores were thus in close contact with 
the glass, and in germination would be nearly in the same 
plane. 
During the afternoon of the following day the spores began 
germinating in the cell culture and one of the spores with 
four germ tubes was photographed. (fig. 1). This same spore 
was photographed on the following day, twenty hours later, 
and is shown in fig. 2. In all cases unless the spore is very 
short and nearly oval a septum appears at the time of ger- 
mination, forming two cells, and at the point of the septum 
the spore becomes constricted even soon after germinating. 
The cell culture now became contaminated with bacteria and 
further growth was impossible. Even at the time of photo- 
graphing no. 2 the bacteria at this place were quite numer- 
ous, and the flocculent matter which clouds one portion of a 
thread and the margins of others is a mass of bacteria (fig. 
The cultures in the Petri dishes were now examined again, 
dilution no. I first. A few colonies were visible to the un- 
aided eye as irregular stellate patches. This examination 
showed that the spores after germination had continued to 
increase in size for some time so that they became several 
times larger than when germination first takes place. One 
of these was photographed with a magnification of about 500 
diameters (fig. 4). With this magnification only the central 
portion of the colony could be shown. The threads radiat- 
ing from the spore turn in various directions so that it is not 
possible to bring them all in the same focal plane. The 
spore was the point which was focussed upon and a few of the 
threads are in the same focal plane and show the proportion- 
ate diameter, the septation and size of the cells. At this 
time the highly refringent granules which appear at the time 
of the germination of the spore and are then comparatively 
