1895. ] Development of Colletotrichum. 309 
small, are now much larger and quite strongly differentiated 
from the hyaline contents. They are quite numerous in the 
enlarged spore and are also present to a less degree in the 
threads. 
At the same time it was observed that the very large ma- 
jority of spores in the same culture, which had failed to ger- 
minate at the time the first germinated, had continued to in- 
crease in size, were once septate, strongly constricted at the 
point of the septum and were richly charged with large highly 
refringent granules. It fact but for the germination of the 
rst spores there could not be determined any difference. 
One of these is shown at fig. 6. The margin was a trifle 
out of the focal plane so that the wall presents a heavier line 
than should be the case. In culture dilution no. 1 none of 
these spores germinated, but in culture dilution no. 2 nearly all 
of them began germinating on the 25th and on the 26th sev- 
eral of these were photographed to show the different results 
which follow. Fig. 7 shows one with only the ordinary germ 
tubes, fig. 8 is developing basidia directly from the spore and 
bearing several spores, others had but few germ tubes ter- 
minated by the oval, dark bodies. It thus seems that these 
spores which do not readily at first germinate become for a 
short time places for the storage of reserve material, and later 
germinate. Whether they would under conditions giving an 
abundance of room produce colonies like the first ones has 
not been determined, for in the present culture they were too 
close together for this result. 
In plate culture no. 2 the older and stellate colonies devel- 
oped a compact stroma at the center which bore numer- 
ous spores with a slight roseate or flesh colored tinge. On 
April 28th four cultures on sterilized bean stems were made 
of this dilution culture in order to study the behavior of these 
normally developed spores in comparison with those which were 
late in germinating and did not develop in this culture many 
Spores. Two of these cultures on bean stems were made by 
transplanting spores from the normally developed colonies, 
and two were made by transplanting some of the agar con- 
taining the second type of spores which were late in germinat- 
in 
May 7th a culture, using numerous spores, was made ina 
Petri dish during the afternoon. This culture was made by 
Pouring a small quantity of agar containing numerous spores 
