1895.] Development of Colletotrichum. 3Ir 
glass was by this time considerably in advance of that of the 
ones used to illustrate these features. In this culture as well 
asin those previously studied, many of the spores did not 
germinate at first, but manifested their activity by absorbing 
nutrient material and assimilating it from the medium, thus 
increasing in size and in the richness of the granules. This 
is also accompanied by the formation of the cross wall mak- 
ing two cells in most cases and also in a greater or lesser con- 
striction of the spore at the middle. These spores begin to 
germinate at varying intervals so that the process of germin- 
ation is going on for several days or even for a week. Forty- 
eight hours after sowing the spores, two of them having recent- 
ly germinated were photographed (fig. 14). | One spore pos- 
sesses two very short germ tubes, one at each end; the other 
spore which was slightly out of the focal plane has not only 
agerm tube from each end but some from the side as well, 
and all but one of the threads are quite long and flexuous. 
Sixty-six hours after sowing two other spores were photo- 
graphed (fig. 15). Here the spores are of considerable size, 
a result of the ‘continuous swelling, and the septum at the 
strongly constricted central portion can easily be seen. 
Cornell University. 
EXPLANATION oF PLaTE XXII. 
- Figures 1 to 15 are photomicrographs of the living organism in the 
oe agar where it was growing. The objects were therefore un- 
Stained. 
n figures 16 and 17 are represented, natural size, the form of the 
mature colonies of two plate cultures. 
