228 . Mr. W. E. Collinge on the 



EXPLANATION OF PLATE XIV. 



Fig. \. Attus maderiana, sp. n., $. Much enlarged. 

 Fig. 2. Marpissa Grantii, sp. n., $ . Much enlarged. 

 Fig. 3. Ditto. Epigyue, 

 Fig, 4. Marpissa ornata, Thorell, 5 • 

 Fig. 5. Ditto. Side view. 

 Fig, 6. Ditto. Epigyne. 



Fig, 7. Misumena Clarkii, sp. n., 5 . Caput, with ocular area. 

 Fig. 8. Ditto. Epigyne. 



Fig. 9. Lithyphantes nobilis, Thorell. Dorsal view of abdomen. 

 Fig. 10. Tegenaria pagana, C. Koch. Epigyne of $ . 

 Fig. 11. Cluhiona decora, Blackwall, cf • Dorsal view of abdomen. 

 Fig. 12. Ditto. Palpus of S • 



Fig, 13. Ariadne maderiana, sp. n., $ (not quite mature). Much 

 enlarged. 



N.B. — The types of the species now described as new are 

 deposited in the British Museum. 



XXVI. — On the Preservation of Teleostean Ova. 

 By Walter E. Collinge, St. Andrews University. 



Between October 1891 and July 1892 upwards of 80,000 

 ova have been examined at the St. Andrews Marine Zoolo- 

 gical Laboratory, comprising some thirty known and four or 

 five unknown species. Upon a large number of these I have 

 made numerous experiments with various preservatives, of 

 which the following notes are an account of the results 

 obtained. 



Killing. 



The most satisfactory results were obtained by adding to a 

 vessel containing the ova, with about an ounce of sea-water, 

 three or four drops of a saturated solution of picric acid, 

 to which had been added 5 per cent, of hydrochloric acid. 

 In this diluted solution they were allowed to remain for not 

 longer than three minutes, during which time they were kept 

 in motion by a pipette. When the ova remained for longer 

 than the time stated, or when the solution was too strong, 

 the yolk was generally ruptured and considerable wrinkling 

 took place in the zona radiata. In other cases the yolk 

 became considerably contracted. Like results ensued if they 

 were not well washed in fresh water before being transferred 

 to the preservative fluid. After washing in dilute alcohol 

 12^-25 per cent., a slight opacity followed. If killed in a 

 saturated solution of corrosive sublimate 6 parts and 3 parts 



