and its Reproductive Cells. 43 



ductive cells, but smaller in size. In two or three instances, but 

 not constantly (fig. 1 d). 



2nd. A single large transparent cell with small granulated 

 nucleus, together with, but much larger than, the reproductive 

 cells. Also not constant (fig. 1 e). 



3rd. A single cell (containing an efi'ete nucleus and several 

 short bacillar filaments) a little larger than the reproductive 

 cells, but present with them. Only seen in one instance. This 

 looked more like an oscillatorial development inside the cell 

 than one of spermatozoids (fig. 9). 



4th. Lastly, I may mention here a spherule like the "fat- 

 globule,^^ which is occasionally discharged from the posterior 

 extremity, and after exit, bursting, shows a distinct capsule, the 

 contents of which separate into a group of minute, swarming 

 molecules, which for some time adhere to the tail of the Amoeba, 

 and at last gradually, one by one, disappear (fig. 2 e). 



But when we consider that Amoeba takes in such a variety of 

 organisms for food, it is evident that we should require in addi- 

 tion to have bodies which have distinct and persistent characters, 

 occurring in the Amoeba almost constantly, to determine those 

 which do and those which do not form a part of the living 

 animal. Therefore I only record the above observations for what 

 they may be worth, and to show how far I have been able to go 

 in the matter of spermatic development in A. princeps. 



Villous appendage (PI. III. fig. 1/). — The villous appendage 

 which marks the posterior end of A. princeps has lately been 

 brought into notice by Dr. Wallich, in the species for which he 

 has proposed the designation of " villosa"^. 



This appendage is figured in my Indian Journal as far back 

 as 1854, also many times since, as before stated, and consists of 

 a number of minute villi, forming a cap-like tuft upon the pos- 

 terior end of the Amoeba. In one instance it appears as if 

 it were composed of several long or large villi covered with 

 smaller ones, thus forming as many tufts as there were large 

 villi (fig. 5 d). Occasionally these tubular or villous extensions 

 of the ectosarc are dilated into cellular forms, and then they give 

 the posterior end of the Amoeba a crenulated aspect (fig. 2 d), 

 while at other times (although this is but seldom) there is little 

 or no trace of them. They are present in the youngest (fig. 3') 

 as well as in the oldest active periods of the Amceba's life, and 

 appear to be always accompanied by finger-like projections of 

 the endosarc into them. When iodine is applied, they spread 

 out into an even edge, like that of the rest of the Amoeba. As 

 Dr. Wallich has stated, they appear to have a roothke or pre- 

 hensile use. Hydra viridis has a tubular structure extending 

 * Annals, he. cit. 



