8 FISH CULTURAL ASSOCIATION. 
first experiment was as follows: By using a small glass syringe 
I injected the milt of the male brook trout into the vent of the 
ripe female brook trout and left it there thirty minutes before 
taking the eggs. The result of this experiment was an impreg- 
nation of 75 per cent. In my second experiment I took the 
spawn from brook trout directly in a vial, and corked tightly, 
taking care that no water was allowed to get in. I then placed 
the vial under water and left it forty minutes, after which brook 
trout milt was put on them and remained in vial thirty minutes, 
the result of which was an impregnation of 75 per cent. 
Third Experiment.—I injected milt of brook trout into ripe 
female, and allowed it to remain fourteen hours before taking. 
15 per cent. of them proved to be good. 
Fourth Experiment.—I injected milt of brook trout into ripe 
female, and allowed it to remain in fish twenty-four hours before 
taking. In this experiment none of the eggs were fertilized. 
Fifth Experiment.—I injected milt of brook trout into ripe 
female, and left it in fish one minute before taking. 40 per cent: 
was impregnated. 
Sixth Experiment.—Took brook trout spawn in vial corked 
tightly, and placed under water for nine hours, after which 
milt was put on them. 15 per cent. of the eggs were impreg- 
nated. 
Seventh Experiment.—Spawn was taken from female brook 
trout three hours after she had died, and milt from live male 
brook trout put onthem. In this experiment 15 per cent. were 
found to be good. 
As all fishculturists know the spawn of brook trout taken in 
the usual way adheres to the pan for from twenty minutes to 
half an hour directly after taking, we tried the experiment of 
putting them directly on the hatching trays within one minute 
after they were taken, and kept the pan in motion so they could 
not stick. The result of this experiment shows that the impreg- 
nation takes place almost instantaneously, as fully 95 per cent. 
were impregnated 
During last summer I spent considerable time on several of 
our inland lakes investigating them, and teaching the local in- 
habitants how to catch the fish with hook and line with which 
