236 
MONOGRAPH OF THE LABOULBEXIACE.E 
» 
ffiised 
o 
The presence of tb 
than Laboulbenia, a 
the latter the punctate appearance peculiar to many species. 
not been definitely determined in genera other 
fib 
has 
although so conspicuous in many species 
they no not seem to be of invariable occurrence. 
The substance of tlie cell walls *as well as of the general envelope give, when 
treated with iodine and sulphuric acid, or with Schultze's chloroiodide of zinc solution, 
no blue color. 
The contents of the cells, especially those of the receptacle, are usually more or less 
characteristic while the plant is alive, and consist of rather dense granular proto- 
plasm, in which, as a rule, certain highly refractive spherical oily masses are conspicu- 
ous. In some instances these masses are few in number, one or more of them being 
often, very large (Plate V, figs. 4 and 5), while ngain they may be more numerous and 
uniform in size, completely filling the cells, as in the case of Laloulbenia Harpall, L. 
Philonthi, and many others. In general, however, they are more or less variable in 
size, and present the appearance indicated in fig. 15, Plate II. In glycerine they soon 
become indistinguishable, and are thus not represented in the accompanying figures, 
■which were drawn, with few exceptions, from glycerine preparations. 
The protoplasm of adjacent cells, the origin of which is the same, is connected by 
picuous strand of the same sub 
ch passes from one cell to the other 
1 
igh a well marked perforation of the cell wall, the connection being demonstrated 
great ease by treatment with potash and subsequent staining (Plate 111, figs. 11- 
Plate II, figs. 16-18). In many instances, also, it may be seen in the living plant; 
of 
r 
F 
This protoplasmic connection is found in all the cell 
le, when this organ is multicellular. In many cas' 
eluding those of the trichogyi 
which the cells had been separated by potash, and the connecting protoplasmic strand 
stretched between them, I have seen a slight enlargement like that indicated in fig. 
1 
Plate III 
recalling the similar structure through which the strands of 
P 
ro- 
toplasm pass in the Florid 
exact nature. 
A siuLde. usi 
I have, however, be 
yet unabl 
d 
ally large, nucleus is found in the contents of 
often readily seen without the use of 
o 
hile in oth 
y to demonstrate, owing to the difficulty 
y cell, 
s by m 
d 
IS 
the cell contents. The 
Pl 
ally experienced in staining 
ly so, and usually 
a large 
nucleolus (Plate V, figs. 20 and 24; Plate I, figs. 7, 15, 16; Plate II, figs. 3 and 13). 
The nuclear changes accompanying cell division have not been determined: but from 
the occurrence of conditions like that represented in 
fi<r. 7, Plate I 
appear 
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