# 
SEDGWICK AND WIXSLOW. 
BACILLUS OF TYrnOTD FETKl}. 
4t 
J 
freezing and thawing, and obtained the fullowing results. The tubes were here im- 
mersed in ice and salt at 
9 
O'^C. 
Frozen 24 hours 
iC 
ii 
cc 
3 days 
4 
a 
5 " 
B. TiTllI. 
In water 
29,780 
1,800 
950 
2,490 
40,890 
Befrozen 3 times 
u 
K 
u 
5 « 
G " 
6 
a 



Frozen 24 hours 
(( 
t< 
(( 
30 
48 
96 
(( 
a 
(( 
B. PRODIGIOSUS. 
In water 
36,410 
41,680 
14,440 
4,850 
330,516 
Refrozcn once 
if 
2 limes 
(( 
4i 
3 
4 
i( 
(( 
2,570 
276 
15 

STAPHYLOCOCCUS PYOGENES AUREUS. 
Frozen 15 minutes 
(4 
2 hours 
(( 
u 
u 
24 
48 
96 
(( 
(( 
(C 
lu water 
52,500 
21,300 
22,690 
6, 4 GO 
6,155 
rco 
111,78 
Kefrozcn once 
K 
(( 
3 times 
4 " 
13,405 
110 

Dr. Pr 
w 
found tliat, with fr 
■'o 
of 
49,280 germs remained 
ty day 
when 
fi 
old 
of innumerable germs 
and dried agar were 
[lis .'jfnphylucocc 
Ijy present, aft 
d, 102,000 g 
appeared entirely after five days. He ultimately drew the following conclusions fro 
these 
experime 
til pathogenic germs 
1. Many bacteria are k 
'J 
freez 
mg 
The vitality of the original culture affects the number so killed. 3 
iiiber killed 
freezii 
with 
species 
4 The number killed increases as the time 
5. The 
terium. 6. Alternate freezino- and 
g is prolonged 
cold varies with 
udividual 
'y 
My fatal 
III", u. 2i.iLeiiiaie ireezmg aiiu liuiwuij^ ab v^ij ^v..^ j 
Dr. Prudden also froze natural waters with their native bacteria for varying period 
d obtained somewhat similar results. He analyzed 270 
pics of New York 
and found an average of 2033 bacteria per cubic centimeter 
The numbers 
were highest 
the 
PP 
layers of 
and bubbly ice, and 
the immediate vicinity of Albany, falling off rapidly in ice five or six miles dov 
river. 
H 
e 
ded that this highly polluted 
of typhoid fever and should 
be 
probably containc 
human body 
gern 
